Description

DEAE Sepharose CL-6B is a robust, beaded anion exchange chromatography medium based on a highly cross-linked 6% agarose matrix. This product is essential for the high-resolution purification of a wide range of biomolecules, including proteins, nucleic acids, and enzymes, offering excellent flow properties and chemical stability. It is a critical tool for researchers and process developers in the biotechnology, pharmaceutical, and life sciences industries who require reliable and scalable separation solutions.

Application

• Protein Purification: Primary purification and intermediate polishing of antibodies, enzymes, and recombinant proteins.
• Nucleic Acid Separation: Fractionation and purification of plasmid DNA, RNA, and oligonucleotides.
• Enzyme Isolation: Capture and purification of specific enzymes from complex biological mixtures.
• Process Development: Scalable method development for downstream bioprocessing in cGMP manufacturing.
• Diagnostic Reagent Preparation: Purification of key components used in diagnostic kits and assays.
• Academic & Industrial Research: A versatile tool for laboratory-scale separation and analysis in life science research.

Basic Information

Product Name	DEAE Sepharose CL-6B
CAS No.	57407-08-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Diethylaminoethyl Sepharose CL-6B; DEAE-Sepharose CL-6B; Sepharose CL-6B DEAE; DEAE Agarose CL-6B; 6% Cross-linked Agarose DEAE; Anion Exchanger Sepharose CL-6B; DEAE Sepharose® CL-6B
EINECS	Contact for details

Quality Control

Our DEAE Sepharose CL-6B is manufactured under a quality management system and undergoes rigorous testing to ensure batch-to-batch consistency for reliable chromatographic performance. Key parameters such as ligand density, particle size distribution, flow rate, and binding capacity are strictly controlled. A Certificate of Analysis (COA) detailing all specifications is provided with each batch to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in 20% ethanol or an appropriate storage solution as specified. Do not freeze. For long-term storage, ensure the medium remains suspended in the recommended preservative solution.

Specification

Item	Specification
Matrix	Cross-linked 6% Agarose
Ligand	Diethylaminoethyl (DEAE)
Particle Size	45-165 μm
Binding Capacity	>110 mg HSA per ml medium
pH Stability	2-14 (long term); 3-12 (working)
Storage Temperature	2-8°C
Preservative	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.