Description

Mtt Formazan CAS NO 57360-69-7 is a vital tetrazolium salt derivative widely used as a chromogenic substrate in cell viability and proliferation assays. Its core value lies in its ability to produce a measurable formazan dye upon reduction by metabolically active cells, providing a reliable colorimetric readout. This makes it an essential reagent for researchers and quality control professionals in the pharmaceutical, biotechnology, and life sciences industries.

Application

• Cell Viability and Proliferation Assays: Primary reagent in MTT assays to measure mitochondrial activity in cell cultures.
• Drug Discovery and Screening: Used in high-throughput screening (HTS) to evaluate the cytotoxic or proliferative effects of novel compounds.
• Cancer Research: Employed to assess the sensitivity of cancer cell lines to chemotherapeutic agents and radiation.
• Toxicology Studies: Applied to determine the in vitro cytotoxicity of chemicals, nanomaterials, and environmental pollutants.
• Immunology Research: Utilized to measure lymphocyte proliferation and immune cell activation in response to stimuli.
• Microbiology: Can be adapted for assessing microbial cell growth and metabolic activity.
• Biomaterial Testing: Used to evaluate the biocompatibility of medical implants and scaffolds by testing cell growth on their surfaces.
• Academic and Industrial R&D: A fundamental tool in basic biological research and applied industrial biotechnology processes.

Basic Information

Item	Detail
Product Name	Mtt Formazan
CAS No.	57360-69-7
Molecular Formula	C18H16N8
Molecular Weight	344.37 g/mol
Synonyms	3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide formazan; Thiazolyl Blue Tetrazolium Bromide Formazan; MTT Formazan; MTT Reduction Product; MTT Tetrazolium Formazan; Formazan, MTT; MTT-F; [3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazol-3-ium] Formazan
EINECS	Contact for details

Quality Control

Our Mtt Formazan is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR), to ensure it meets the high standards required for sensitive biological assays. Certificates of Analysis (COA) with lot-specific data are available upon request to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term stability, consider storage at 2-8°C. Keep the container tightly sealed to minimize exposure to air and moisture.

Specification

Item	Specification
Appearance	Dark blue to purple crystalline powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.