Description

Putidaredoxin is a specialized, iron-sulfur redox protein that serves as a critical electron transfer component in bacterial monooxygenase systems. Its primary value lies in its role as a biological catalyst, facilitating specific hydroxylation reactions essential for metabolic pathways and bioremediation processes. This compound is particularly vital for researchers and manufacturers in the fields of enzymology, biochemical research, and the development of biocatalysts for pharmaceutical synthesis and environmental applications.

Application

• Biocatalyst Research & Development: As an essential electron donor in cytochrome P450cam (CYP101) systems for studying stereospecific hydroxylation mechanisms.
• Enzymology Studies: Used in vitro to investigate electron transfer kinetics and protein-protein interactions within bacterial monooxygenase complexes.
• Pharmaceutical Intermediate Synthesis: Potential application in enzymatic cascades for the production of chiral intermediates, leveraging its role in camphor degradation pathways.
• Bioremediation Model Systems: Employed in research focused on microbial degradation of hydrocarbons and environmental pollutants.
• Protein Biochemistry: Serves as a model protein for studying the structure and function of [2Fe-2S] cluster-containing ferredoxins.
• Educational & Reference Standard: Used in academic and industrial laboratories as a high-purity standard for analytical methods and experimental protocols.

Basic Information

Product Name	Putidaredoxin
CAS No.	57087-75-9
Molecular Formula	C~xH~yN~zO~wS2Fe2*
Molecular Weight	~12,500 Da (Protein) / Contact for exact monomeric details
Synonyms	Putidaredoxin; Pdx; Pseudomonas putida ferredoxin; Camphor 5-monooxygenase ferredoxin; CYP101 ferredoxin; Electron transfer protein for camphor hydroxylase; [2Fe-2S] ferredoxin (putidaredoxin-type); EC 1.18.1.4 (associated reductase component)
EINECS	Contact for details

*The exact molecular formula varies based on the specific protein sequence and expression system. Contact us for detailed structural information.

Quality Control

Our Putidaredoxin is produced and purified under controlled conditions to ensure high biological activity and structural integrity. Each batch undergoes rigorous analytical testing, including assessment of purity (via SDS-PAGE and HPLC), iron-sulfur cluster content, and specific activity in standard electron transfer assays. Certificates of Analysis (COA) detailing these parameters are available upon request to support your research and development quality standards.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below in suitable aliquots. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed under inert atmosphere or desiccated conditions after opening to maintain activity.

Specification

Item	Specification
Appearance	Brownish-red, lyophilized powder or solution
Identification (UV-Vis)	Characteristic absorption spectrum for [2Fe-2S] cluster
Purity (SDS-PAGE)	≥ 90% (Single band)
Purity (HPLC)	≥ 95%
Protein Content (Bradford/Lowry)	≥ 90% of dry weight
Specific Activity	Contact for details
Iron Content	2.0 ± 0.3 mol per mol protein
Inorganic Sulfide Content	2.0 ± 0.3 mol per mol protein
Solubility	Soluble in appropriate buffers (e.g., Tris-HCl, phosphate)
Endotoxin Level	< 1.0 EU/mg (if required)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.