Description

2,3-Di(4-Biphenylyl)-5-Phenyl-2H-Tetrazolium Chloride is a specialized tetrazolium salt widely recognized for its role as a vital redox indicator in biochemical assays. This compound matters because it provides a colorimetric endpoint for detecting cellular metabolic activity, particularly in cell viability and cytotoxicity testing. It is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors who require reliable and sensitive reagents for enzymatic analysis.

Application

• Cell Viability and Proliferation Assays (e.g., MTT Assay): Used as a substrate for mitochondrial dehydrogenases in living cells to formazan dyes.
• Microbiological Testing: Employed as an indicator for bacterial growth and metabolic activity in various culture media.
• Enzymatic Activity Detection: Serves as an electron acceptor in dehydrogenase enzyme assays for biochemical research.
• Toxicology Screening: Applied in in vitro models to assess the cytotoxic effects of chemical compounds and pharmaceuticals.
• Diagnostic Reagent Formulation: A key component in kits for clinical diagnostics and research use only (RUO) products.
• Material Science Research: Investigated for potential applications in electrochemistry and sensor development due to its redox properties.

Basic Information

Product Name	2,3-Di(4-Biphenylyl)-5-Phenyl-2H-Tetrazolium Chloride
CAS No.	56862-91-0
Molecular Formula	C₃₁H₂₂ClN₄
Molecular Weight	485.98 g/mol
Synonyms	2,3-Bis(4-biphenylyl)-5-phenyl-2H-tetrazolium chloride; 2,3-Di(4-biphenylyl)-5-phenyltetrazolium chloride; 2,3-Di(biphenyl-4-yl)-5-phenyl-2H-tetrazol-3-ium chloride; Tetrazolium Red Chloride; TV; TV Chloride; BPTT; 2,3,5-Triphenyltetrazolium chloride derivative
EINECS	Contact for details

Quality Control

Our 2,3-Di(4-Biphenylyl)-5-Phenyl-2H-Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by FT-IR and NMR, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals (as Pb)	≤ 20 ppm
Solubility Test	Clear, yellow solution in DMSO or DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.