Description

p-Nitrophenyl β-D-Maltoside is a chromogenic substrate specifically designed for the detection and quantification of α-amylase enzyme activity. This compound matters because it provides a reliable, colorimetric readout essential for research, diagnostic, and quality control applications where precise enzyme measurement is critical. It is primarily needed by professionals in the biotechnology, pharmaceutical, and food science industries for enzymatic assays and diagnostic kit development.

Application

• α-Amylase Activity Assays: Primary substrate for the spectrophotometric determination of α-amylase in clinical and research laboratories.
• Diagnostic Kit Manufacturing: Key component in the production of reagent kits for the diagnosis of pancreatic disorders and other conditions affecting amylase levels.
• Biochemical Research: Used in enzymology studies to investigate enzyme kinetics, inhibition, and the mechanism of action of α-amylase.
• Food & Beverage Industry Analysis: Employed in quality control to measure diastatic activity in brewing, baking, and starch processing.
• Pharmaceutical Development: Utilized in screening for potential α-amylase inhibitors relevant to diabetes management.
• Microbiology: Applied in microbial identification protocols that rely on starch hydrolysis as a diagnostic characteristic.

Basic Information

Product Name	p-Nitrophenyl β-D-Maltoside
CAS No.	56846-39-0
Molecular Formula	C18H25NO13
Molecular Weight	463.39 g/mol
Synonyms	4-Nitrophenyl β-D-maltoside; p-Nitrophenyl β-maltoside; 4-Nitrophenyl β-maltoside; PNPM; β-4-Nitrophenyl maltoside; 1-O-(4-Nitrophenyl)-β-D-maltoside; 4-Nitrophenyl-β-D-maltopyranoside; Amylase substrate
EINECS	Contact for details

Quality Control

Our p-Nitrophenyl β-D-Maltoside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive enzymatic applications. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This product is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.