Description

Uridine-5 6-3H CAS NO 56654-40-1 is a high-specific-activity tritium-labeled nucleoside, essential for advanced biochemical and pharmacological research. This compound serves as a critical tracer for studying nucleic acid metabolism, cellular uptake mechanisms, and the pharmacokinetics of nucleoside-based therapeutics. It is primarily utilized by research scientists and quality control laboratories in the pharmaceutical, biotechnology, and academic sectors for radioligand binding assays, autoradiography, and metabolic pathway analysis.

Application

• Biochemical Research: As a radiolabeled tracer for investigating pyrimidine metabolism, RNA synthesis, and nucleoside salvage pathways.
• Pharmacology & Drug Development: For studying the transport, cellular incorporation, and catabolism of nucleoside analog drugs in preclinical models.
• Molecular Biology: Use in autoradiography and other detection methods to visualize and quantify RNA in complex biological samples.
• Enzyme Assays: Serving as a substrate for enzymes such as uridine kinase and other nucleoside-metabolizing enzymes to determine kinetic parameters.
• Diagnostic Probe Development: Potential use in the development of diagnostic assays for monitoring disease states related to nucleotide imbalance.
• Quality Control (QC): Employed as an internal standard or reference material in analytical methods within pharmaceutical QC laboratories.

Basic Information

Product Name	Uridine-5 6-3H
CAS No.	56654-40-1
Molecular Formula	C9H113H N2O6
Molecular Weight	Contact for details
Synonyms	5,6-3H-Uridine; [5,6-3H]Uridine; Tritiated Uridine; Uridine (5,6-tritium); Uridine-5,6-t; 1-β-D-Ribofuranosyl-5,6-tritiouracil; 2,4(1H,3H)-Pyrimidinedione, 5,6-tritio-1-β-D-ribofuranosyl-
EINECS	Contact for details

Quality Control

Every batch of Uridine-5 6-3H is manufactured and analyzed under strict quality systems to ensure exceptional purity and specific activity. Our products undergo rigorous quality testing, including radiochemical purity analysis by HPLC, to ensure compliance with research-grade standards. A comprehensive Certificate of Analysis (COA) detailing specific activity, purity, solvent composition, and analytical methods is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dedicated freezer for radioactive materials. Due to its nature as a highly volatile radionuclide (tritium), ensure containers are securely sealed and stored in a well-ventilated area according to local radiation safety regulations. Keep away from heat and open flame.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms
Radiochemical Purity (HPLC)	≥ 97%
Specific Activity	20-40 Ci/mmol
Chemical Purity (HPLC)	≥ 95%
Solvent	Ethanol:Water (7:3 v/v)
Concentration	1 mCi/mL

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.