Description

D-Luciferin is a high-purity, synthetic substrate essential for bioluminescence reactions. Its core value lies in enabling highly sensitive, real-time detection and quantification of biological processes in research and drug discovery. This compound is a critical reagent for scientists and professionals in the life sciences, pharmaceutical development, and molecular diagnostics industries. D-Luciferin CAS NO 55963-96-7 is the standard for in vitro and in vivo imaging applications.

Application

• Bioluminescence Imaging (BLI): Primary substrate for non-invasive, real-time monitoring of tumor growth, metastasis, and treatment efficacy in live animal models.
• Reporter Gene Assays: Used with luciferase genes (e.g., firefly luciferase) for high-throughput screening (HTS) of drug candidates, gene expression studies, and promoter activity analysis.
• Cell Viability & Proliferation Assays: Enables ATP quantification as a measure of cellular metabolic activity and cytotoxicity.
• Molecular Diagnostics: Serves as a key component in diagnostic kits for detecting specific pathogens or biomarkers via bioluminescent signals.
• Biosensor Development: Integral to the design of sensitive biosensors for environmental monitoring, food safety testing, and clinical diagnostics.
• Academic & Basic Research: Fundamental tool for studying circadian rhythms, protein-protein interactions, and intracellular signaling pathways.

Basic Information

Product Name	D-Luciferin
CAS No.	55963-96-7
Molecular Formula	C11H8N2O3S2
Molecular Weight	280.32 g/mol
Synonyms	D-(-)-Luciferin; Firefly Luciferin; (4S)-4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid; Beetle Luciferin; D-Luciferin free acid; LH2; Luciferin (Firefly); D-Luciferin, Potassium Salt (for related salt form)
EINECS	Contact for details

Quality Control

Our D-Luciferin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and MS for identity verification, to ensure it meets the stringent requirements for research and assay development. Certificates of Analysis (COA) with lot-specific data are provided to guarantee reproducibility and performance in your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%
Bioluminescent Activity	Meets performance standards

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.