Description

Keratanase is a highly specific endo-β-galactosidase enzyme that catalyzes the hydrolysis of keratan sulfate, a key glycosaminoglycan. This specificity makes it an indispensable tool for structural analysis and modification of complex carbohydrates in research and industrial applications. It is primarily utilized by biotechnology firms, pharmaceutical R&D departments, and diagnostic manufacturers for glycobiology research, biopharmaceutical characterization, and the development of therapeutic agents.

Application

• Glycobiology Research: Used for the controlled degradation and sequencing of keratan sulfate to study its structure-function relationships in cellular processes.
• Biopharmaceutical Analysis: Employed in the characterization of glycosylation patterns on monoclonal antibodies and other protein-based therapeutics to ensure product consistency and efficacy.
• Diagnostic Reagent Production: Serves as a key component in enzymatic assay kits for the detection and quantification of specific glycosaminoglycans in clinical samples.
• Therapeutic Development: Investigated for use in enzyme replacement therapies and as a processing aid in the production of bioactive oligosaccharides.
• Biomaterial Modification: Applied to modify the surface properties of biomaterials by cleaving specific carbohydrate motifs, altering cell-material interactions.

Basic Information

Product Name	Keratanase
CAS No.	55072-01-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Keratan sulfate endo-1,4-β-galactosidase; Endo-β-galactosidase; Keratanase I; Keratan sulfate-degrading enzyme; Pseudomonas sp. keratanase; EC 3.2.1.103
EINECS	Contact for details

Quality Control

Our Keratanase is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high enzymatic activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and absence of contaminating enzymatic activities. We adhere to relevant guidelines for enzyme production to support research and industrial applications requiring reliable performance.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Hydrolyzes keratan sulfate specifically
Specific Activity	≥ X units/mg protein (Contact for details)
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	Contact for details
Contaminating Activities	Free of detectable protease, chondroitinase, and hyaluronidase activities
Solubility	Soluble in water or appropriate buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.