Description

n-Tris(Hydroxymethyl)Methyl-4-Aminobutanesulfonic Acid is a high-purity zwitterionic biological buffer essential for maintaining stable pH in biochemical and diagnostic applications. Its primary value lies in its excellent buffering capacity in the physiological pH range, minimal interference with biological processes, and high solubility in aqueous solutions. This makes it a critical reagent for researchers and manufacturers in molecular biology, cell culture, diagnostic kit formulation, and pharmaceutical development who require precise and consistent pH control.

Application

• Biochemical & Molecular Biology Research: As a key component in electrophoresis buffers (e.g., TABS-PAGE), PCR master mixes, and enzyme reaction systems to ensure optimal activity and stability.
• Cell Culture & Tissue Engineering: Used in media formulations to provide a stable, non-toxic pH environment for mammalian, bacterial, and yeast cell growth.
• Diagnostic Reagent Manufacturing: Incorporated into buffers for clinical diagnostic assays, immunoassays (ELISA), and biosensor platforms to guarantee test accuracy and reproducibility.
• Pharmaceutical Development: Serves as an excipient or buffering agent in the formulation of protein therapeutics, vaccines, and other biologics to enhance product stability.
• Chromatography: Utilized as a mobile phase modifier or running buffer in ion-exchange and size-exclusion chromatography to improve separation efficiency.
• Cosmetic & Personal Care: Acts as a pH stabilizer and conditioning agent in high-performance skincare and haircare formulations.

Basic Information

Product Name	n-Tris(Hydroxymethyl)Methyl-4-Aminobutanesulfonic Acid
CAS No.	54960-65-5
Molecular Formula	C8H19NO7S
Molecular Weight	273.30 g/mol
Synonyms	TABS; Tris-Aminobutanesulfonic Acid; 4-Amino-2-hydroxy-1,1-bis(hydroxymethyl)ethylbutane-1-sulfonic Acid; 2-{[1,3-Dihydroxy-2-(hydroxymethyl)propan-2-yl]amino}ethane-1-sulfonic Acid; N-Tris(Hydroxymethyl)Methyl-4-Aminobutanesulfonate; TABS Buffer; Good's Buffer TABS
EINECS	259-423-4

Quality Control

Our n-Tris(Hydroxymethyl)Methyl-4-Aminobutanesulfonic Acid is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure it meets high-purity standards suitable for sensitive life science applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are provided with every shipment. We can supply grades compliant with relevant industry guidelines for research and manufacturing use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated area at room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture, which can affect purity and performance.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 99.0%
pH (1% Solution)	10.5 - 11.5
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals (as Pb)	≤ 10 ppm
UV Absorbance (260 nm)	≤ 0.05
Endotoxin (LAL)	< 0.1 EU/mg (for applicable grades)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.