Description

3-(3-Maleimidopropylcarbamoyl)-Proxyl is a stable nitroxide radical spin label featuring a maleimide functional group, enabling site-specific covalent conjugation to thiol groups in biomolecules. This compound is critical for advanced research in structural biology and biophysics, providing a powerful tool for probing protein dynamics, conformation, and interactions via Electron Paramagnetic Resonance (EPR) spectroscopy. It is primarily utilized by research institutions, pharmaceutical R&D departments, and diagnostic developers working on protein structure-function studies and biomolecular assay development.

Application

• Site-Directed Spin Labeling (SDSL) for EPR Spectroscopy: Covalently attaches to cysteine residues in proteins to study conformational changes, folding, and dynamics.
• Protein-Protein Interaction Studies: Used as a molecular probe to investigate binding interfaces and complex formation.
• Membrane Protein Research: Labels membrane-embedded proteins to analyze topology and lipid interactions.
• Nucleic Acid Labeling: Can be used to modify thiol-modified oligonucleotides for studying nucleic acid structure and protein-nucleic acid complexes.
• Bioconjugation for Diagnostic Assays: Serves as a stable radical tag for developing novel EPR-based immunoassays or biosensors.
• Polymer and Material Science: Acts as a radical initiator or probe for studying polymer kinetics and radical processes.

Basic Information

Product Name	3-(3-Maleimidopropylcarbamoyl)-Proxyl
CAS No.	54135-55-6
Molecular Formula	C15H22N3O4
Molecular Weight	308.36 g/mol
Synonyms	3-Maleimido-PROXYL; 3-Maleimidopropylcarbamoyl-Proxyl; Maleimido-Proxyl; 3-(3-Maleimidopropanoylamino)-2,2,5,5-tetramethyl-1-pyrrolidinyloxy; 3-Maleimido-2,2,5,5-tetramethyl-1-pyrrolidinyloxy; PROXYL maleimide; 3-Maleimido-PROXYL spin label; 2,2,5,5-Tetramethyl-1-pyrrolidinyloxy-3-maleimidopropionamide
EINECS	Contact for details

Quality Control

Our 3-(3-Maleimidopropylcarbamoyl)-Proxyl is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and radical content. We adhere to relevant quality management standards to support our global customer base in academic and industrial research.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation. Keep the container in a dry, cool, and well-ventilated area. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Radical Content (EPR)	≥ 90%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.