Description

4-Methylumbelliferyl-N,N',N''-Triacetyl-β-Chitotrioside is a specialized fluorogenic substrate designed for the sensitive detection and quantification of chitinase enzyme activity. This compound matters because it provides a reliable, high-throughput method for enzymatic research, offering a clear fluorescent signal upon cleavage. It is primarily needed by researchers and quality control laboratories in the life sciences, pharmaceutical, and agricultural biotechnology sectors for studying chitin degradation pathways and screening for enzyme inhibitors or activators.

Application

• Chitinase Enzyme Assays: Primary substrate for the fluorometric determination of endo- and exo-chitinase activity in research and diagnostic kits.
• High-Throughput Screening (HTS): Enables rapid screening of compound libraries for chitinase inhibitors or activators in drug discovery programs, particularly for antifungal and insecticide development.
• Biochemical Research: Used to study the kinetics, mechanism, and specificity of chitinolytic enzymes from various biological sources (e.g., fungi, bacteria, plants, insects).
• Agricultural Biotechnology: Applied in research focused on plant defense mechanisms against fungal pathogens and the development of transgenic crops with enhanced chitinase expression.
• Diagnostic Development: Serves as a key component in developing clinical or environmental diagnostic tests for fungal infections based on chitinase detection.
• Quality Control: Used in the QC of enzyme preparations and related bioproducts to ensure consistent and specified chitinase activity.

Basic Information

Product Name	4-Methylumbelliferyl-N,N',N''-Triacetyl-β-Chitotrioside
CAS No.	53643-13-3
Molecular Formula	C38H47NO23
Molecular Weight	861.78 g/mol
Synonyms	4-MU-(GlcNAc)3; 4-Methylumbelliferyl β-D-N,N',N''-triacetylchitotrioside; 4-MU-Chitotrioside; MUF-(GlcNAc)3; 4-Methylumbelliferyl tri-N-acetyl-β-chitotrioside; Fluorogenic chitotrioside substrate; Chitinase substrate III
EINECS	Contact for details

Quality Control

Our 4-Methylumbelliferyl-N,N',N''-Triacetyl-β-Chitotrioside is produced and tested under strict quality management protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant industry standards for biochemical reagents.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Specific Rotation	Contact for details
Fluorogenic Activity	Contact for details
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.