Description

Aerolysin is a potent, pore-forming protein toxin primarily produced by the bacterium *Aeromonas hydrophila*. This compound is of significant interest in biochemical and pharmacological research due to its unique mechanism of action, which involves binding to cell membranes and forming transmembrane channels, leading to cell lysis. It serves as a critical tool for researchers in structural biology, immunology, and the development of novel therapeutic strategies, including targeted drug delivery systems and cancer research.

Application

• Biochemical Research: Used as a model protein to study the mechanisms of pore-forming toxins, membrane protein insertion, and oligomerization.
• Cell Biology Studies: Employed to create controlled pores in cell membranes for permeability assays, intracellular delivery of molecules, and studying cell death pathways (oncosis).
• Immunology & Vaccine Development: Investigated for its role as a virulence factor and its potential use in constructing toxoid vaccines against *Aeromonas* infections.
• Drug Delivery Systems: Explored for engineering targeted cytolysins or "molecular drills" for selective destruction of cancer cells or for triggering the release of drugs from liposomes.
• Biosensor Development: Utilized in the design of membrane-based sensing platforms that detect specific ligands by triggering pore formation.
• Structural Biology: A key subject for X-ray crystallography and cryo-electron microscopy studies to understand toxin-receptor interactions and pore architecture.

Basic Information

Product Name	Aerolysin
CAS No.	53126-24-2
Molecular Formula	Contact for details
Molecular Weight	Approx. 52 kDa (monomer)
Synonyms	Aerolysin; Aeromonas hydrophila aerolysin; Proaerolysin; Aerolysin precursor; AerA toxin; Pore-forming toxin Aerolysin; Cytolytic enterotoxin
EINECS	Contact for details

Quality Control

Our Aerolysin is produced and handled under stringent quality protocols to ensure batch-to-batch consistency and biological activity. Each lot is characterized for purity, identity, and functional activity. Certificates of Analysis (COA) are provided, detailing specifications such as purity by SDS-PAGE, endotoxin levels, and functional hemolytic activity assays. We adhere to relevant quality management standards to support research and development applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -80°C to minimize freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use. Store away from incompatible materials.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Corresponds to monomer (~52 kDa) and proaerolysin (~54 kDa)
Purity (SDS-PAGE)	> 90%
Protein Content (Bradford)	≥ 1 mg/vial
Biological Activity (Hemolytic Assay)	Defined HC50 value per batch
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.