Description

Azocoll Substrate is a specialized, dye-impregnated collagen preparation used as a chromogenic substrate for the detection and quantification of proteolytic enzyme activity. Its value lies in providing a standardized, sensitive, and convenient assay system for measuring protease concentration and kinetics. This product is essential for researchers and quality control laboratories in the biotechnology, pharmaceutical, diagnostic, and academic research sectors who require reliable tools for enzyme characterization and process monitoring.

Application

• Protease Activity Assays: Quantitative measurement of collagenase, trypsin, pepsin, and other proteolytic enzymes.
• Enzyme Kinetics Studies: Determination of Michaelis-Menten constants (Km and Vmax) for proteases.
• Biopharmaceutical Quality Control: Monitoring and validation of downstream purification processes involving enzymatic steps.
• Diagnostic Reagent Manufacturing: A key component in kits for clinical diagnostics related to tissue remodeling and disease states.
• Microbiological Research: Assaying for microbial proteases as virulence factors or industrial enzymes.
• Tissue Culture & Cell Biology: Studies involving extracellular matrix degradation and cell migration.

Basic Information

Product Name	Azocoll Substrate
CAS No.	53092-90-3
Molecular Formula	N/A (Prepared Collagen Complex)
Molecular Weight	N/A
Synonyms	Azocoll; Azo-Collagen; Collagen, azodye-impregnated; Diazotized Collagen Substrate; Protease Substrate Azocoll; CAS 53092-90-3
EINECS	Contact for details

Quality Control

Our Azocoll Substrate is manufactured under a controlled quality system to ensure batch-to-batch consistency and performance reliability. Each lot is tested for key parameters including protease digestion sensitivity, particle size distribution, and dye release characteristics to guarantee optimal assay performance. A comprehensive Certificate of Analysis (COA) detailing purity, performance specifications, and test results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. For long-term stability, consider storage under desiccant.

Specification

Item	Specification
Appearance	Brownish-orange powder or granules
Identification (IR)	Conforms to standard
Protease Sensitivity	Releases soluble dye upon digestion by standard collagenase
Particle Size	50-150 mesh (Specify grade upon inquiry)
Moisture Content	< 8.0% (Karl Fischer)
Assay (Dye Content)	≥ 0.4 mmol/g (or per grade specification)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.