Description

Endoglycosidase D is a highly specific enzyme that catalyzes the hydrolysis of the glycosidic bond within oligosaccharide chains, particularly targeting the chitobiose core of N-linked glycans. This precise activity makes it an indispensable tool for the structural analysis and sequencing of complex carbohydrates in glycoproteins. It is essential for researchers and manufacturers in the fields of glycobiology, biopharmaceutical development, and diagnostic reagent production. The product is supplied as a lyophilized powder, ensuring stability and ease of reconstitution for laboratory and process-scale use.

Application

• Glycoprotein Structural Analysis: Used for the controlled deglycosylation of N-linked glycans to determine glycan structure, branching, and composition.
• Biopharmaceutical Characterization: Critical for the analysis of therapeutic monoclonal antibodies (mAbs) and other glycoprotein drugs to ensure batch-to-batch consistency and efficacy.
• Enzymatic Synthesis: Employed in the chemoenzymatic synthesis of defined glycan structures for research and development of glycoconjugate vaccines.
• Diagnostic Development: Used in the preparation of glycoprotein standards and reagents for clinical diagnostic assays.
• Food & Beverage Analysis: Applied in the study of glycoproteins in food products for quality control and nutritional research.
• Academic & Industrial Research: A fundamental reagent in glycoscience research for investigating protein-glycan interactions and cellular signaling pathways.

Basic Information

Item	Detail
Product Name	Endoglycosidase D
CAS No.	52769-51-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Endo D; Endo-β-N-acetylglucosaminidase D; N-Glycanase D; Peptide-N4-(N-acetyl-β-glucosaminyl)asparagine amidase D; Glycopeptidase D; EC 3.2.1.96; Endoglycosidase from *Diplococcus pneumoniae*
EINECS	Contact for details

Quality Control

Our Endoglycosidase D is produced under stringent quality management systems. Each lot undergoes rigorous testing to ensure high specific activity, purity, and absence of contaminating protease or other glycosidase activities. A comprehensive Certificate of Analysis (COA) is provided, detailing specifications for purity (by SDS-PAGE), specific activity, protein concentration, and stability. Our quality commitment supports applications requiring reproducible and reliable enzymatic performance in research and regulated environments.

Storage

Preserve in a tightly closed container, protected from light. For long-term storage, keep the lyophilized powder at -20°C. Upon reconstitution, aliquot and store at -20°C to -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the vial is equilibrated to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Cleaves N-linked glycans at the chitobiose core
Purity (SDS-PAGE)	> 95%
Specific Activity	> 50,000 units/mg protein
Protein Concentration	Contact for details
Contaminants (Protease)	Not detectable
Contaminants (Other Glycosidases)	Not detectable
Solubility	Soluble in water or appropriate buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.