Description

Heparinase is a specific enzyme that catalyzes the depolymerization of heparin and heparan sulfate glycosaminoglycans. This product is essential for research and industrial processes requiring the controlled degradation or analysis of these complex polysaccharides. It is a critical reagent for pharmaceutical development, quality control in heparin production, and biochemical research in academia and biotechnology. The CAS number for this compound is 52227-76-6.

Application

• Heparin and Low Molecular Weight Heparin (LMWH) Analysis: Used to depolymerize heparin for structural analysis, sequence determination, and quality control testing of pharmaceutical heparin products.
• Biopharmaceutical Processing: Employed in the downstream processing of biotherapeutics to remove contaminating heparan sulfate or heparin from cell culture media or final products.
• Biomedical Research: A vital tool for studying the role of heparan sulfate proteoglycans in biological processes such as cell signaling, growth factor binding, and viral infection.
• Diagnostic Kit Manufacturing: Incorporated into diagnostic assays and kits designed to measure heparin levels or activity in clinical samples.
• Glycobiology Studies: Used to prepare defined oligosaccharide fragments from heparin/heparan sulfate for functional and structural studies.
• Enzyme Activity Assays: Serves as a standard or reagent in enzymatic activity tests and for the development of new analytical methods.

Basic Information

Product Name	Heparinase
CAS No.	52227-76-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Heparinase I; Heparin lyase I; Heparin eliminase; Flavobacterium heparinum heparinase; EC 4.2.2.7; Heparinase (Flavobacterium heparinum); Heparan sulfate lyase I
EINECS	Contact for details

Quality Control

Our Heparinase is produced and tested under stringent quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and functional consistency. Certificates of Analysis (COA) detailing purity, activity (IU/mg), and absence of contaminating activities are provided to guarantee it meets the exacting standards required for pharmaceutical and research applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture uptake. For optimal performance, prepare working aliquots to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (Activity)	Conforms
Specific Activity	≥ 100 IU/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	Reported on COA
Endotoxin Level	< 1.0 EU/μg
Solubility	Soluble in appropriate aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.