Description

Bis(4-Methylumbelliferyl)Phosphate is a specialized fluorogenic substrate and biochemical reagent. This compound is critical for the sensitive detection and quantification of enzymatic activity, particularly for phosphatases and phosphodiesterases. It is an essential tool for researchers and quality control laboratories in the pharmaceutical, biotechnology, and diagnostic industries, where precise enzyme kinetics and high-throughput screening are required.

Application

• Enzyme Assay Substrate: Primary fluorogenic substrate for alkaline phosphatase (ALP), acid phosphatase, and other phosphatase enzymes in research and diagnostic kits.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify enzyme inhibitors or activators for therapeutic target validation.
• Biochemical Research: Tool for studying enzyme kinetics, mechanism of action, and phosphate metabolism in cellular and molecular biology.
• Diagnostic Manufacturing: Key component in the formulation of clinical chemistry reagents for automated analyzers to measure enzyme levels.
• Cell-Based Assays: Employed in live-cell imaging and flow cytometry to monitor phosphatase activity as a cellular signaling reporter.
• Quality Control Testing: Used in biopharmaceutical manufacturing for in-process testing and purity assessment of enzyme-based products.

Basic Information

Product Name	Bis(4-Methylumbelliferyl)Phosphate
CAS No.	51379-07-8
Molecular Formula	C20H14O9P
Molecular Weight	438.30 g/mol
Synonyms	4-Methylumbelliferyl phosphate disodium salt (common form); Bis(4-methylumbelliferyl) phosphate; Di(4-methylumbelliferyl) phosphate; 4-MU phosphate; 4-Methylumbelliferyl phosphate dimer; Phosphoric acid bis(4-methyl-2-oxo-2H-chromen-7-yl) ester; Fluorogenic phosphatase substrate; ALP substrate
EINECS	Contact for details

Quality Control

Our Bis(4-Methylumbelliferyl)Phosphate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your regulatory and research documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Fluorogenic Activity	Conforms to reference standard
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.