Description

Actin From Rabbit Muscle CAS NO 51005-14-2 is a highly purified, globular protein essential for fundamental cellular processes. It serves as a critical reagent for in vitro studies of cytoskeletal dynamics, muscle contraction, and molecular motor function. This product is indispensable for researchers and developers in the fields of biochemistry, cell biology, and pharmaceutical discovery, providing a reliable and consistent substrate for assays and experimental models.

Application

• Biochemical Research: Fundamental studies of actin polymerization, filament formation (F-actin), and cytoskeleton assembly/disassembly kinetics.
• Drug Discovery & Screening: Target protein for high-throughput screening (HTS) assays to identify novel compounds affecting cytoskeletal integrity for therapeutic applications.
• Molecular Motor Assays: Essential component in motility assays to study the function of myosin, kinesin, and dynein motor proteins.
• In Vitro Diagnostics (IVD): Used as a control or standard in diagnostic test development for muscle-related disorders.
• Biophysics Studies: Substrate for single-molecule studies and force measurements using techniques like optical tweezers and atomic force microscopy (AFM).
• Educational & Teaching Kits: Key component in advanced laboratory kits for university-level courses in molecular and cell biology.

Basic Information

Product Name	Actin From Rabbit Muscle
CAS No.	51005-14-2
Molecular Formula	Contact for details
Molecular Weight	~42 kDa (monomeric G-actin)
Synonyms	Actin, Rabbit Muscle; G-Actin; Actin, Skeletal Muscle; Globular Actin; Actin Protein; 42 kDa Actin; Cytoskeletal Actin; Muscle Actin; Actin Monomer
EINECS	Contact for details

Quality Control

Our Actin From Rabbit Muscle is produced under stringent conditions to ensure high biological activity and purity, suitable for sensitive research applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, activity, and absence of contaminating proteins. We adhere to rigorous quality testing protocols to guarantee batch-to-batch consistency and performance reliability.

Storage

Preserve in a tightly closed container, protected from light. Store frozen at -20°C to -80°C in small aliquots to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed properly after use. For long-term stability, store under recommended frozen conditions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder or clear solution
Purity (SDS-PAGE)	≥ 95%
Protein Concentration	As per COA (typically 1-10 mg/mL)
Biological Activity (Polymerization)	≥ 90% polymerizable
Endotoxin Level	< 1.0 EU/mg (for cell culture grades)
Buffer Composition	5 mM Tris-HCl, 0.2 mM CaCl₂, 0.2 mM ATP, pH 8.0 (or as specified)
Identification (SDS-PAGE/Western Blot)	Complies

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.