Description

Aequorin Type III is a purified photoprotein isolated from the jellyfish *Aequorea victoria*, widely recognized for its role as a highly sensitive calcium indicator. Its core value lies in enabling precise, real-time measurement of intracellular calcium ion (Ca²⁺) fluxes, a critical parameter in numerous biological research and diagnostic applications. This product is essential for researchers and developers in the fields of cell biology, neuroscience, pharmacology, and high-throughput screening, where understanding calcium signaling pathways is paramount.

Application

• Intracellular Calcium Imaging: Primary tool for visualizing and quantifying dynamic changes in cytosolic Ca²⁺ concentration in live cells.
• Biosensor Development: Key component in the construction of genetically encoded calcium indicators (GECIs) for long-term, non-invasive cellular studies.
• Neurobiological Research: Monitoring neuronal activity, synaptic transmission, and calcium-dependent signaling cascades in neural circuits.
• Drug Discovery & Toxicology Screening: Used in cell-based assays to evaluate compound effects on calcium homeostasis and identify potential drug candidates or toxicants.
• Cardiovascular & Muscle Physiology: Studying excitation-contraction coupling and calcium dynamics in cardiac myocytes and skeletal muscle cells.
• Diagnostic Kit Component: Employed in vitro for the development of sensitive diagnostic assays measuring calcium-related biomarkers.

Basic Information

Product Name	Aequorin Type III
CAS No.	50934-79-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Aequorin (from Aequorea); Photoprotein Aequorin; Aequorin Type 3; Ca²⁺-sensitive photoprotein; Aequorin complex; Bioluminescent photoprotein; Aequorin III; Aequorin from *Aequorea victoria*
EINECS	Contact for details

Quality Control

Our Aequorin Type III is produced and handled under stringent quality protocols to ensure batch-to-batch consistency and optimal biological activity. Each lot undergoes rigorous testing, including assessment of luminescent activity, purity profile, and functional performance in standard calcium response assays. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and storage buffer composition is provided with every shipment to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C to -80°C. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation. Handle under appropriate laboratory conditions.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Purity (SDS-PAGE)	> 95%
Specific Activity	Contact for details
Calcium Response Range	~ 0.1 µM to > 100 µM Ca²⁺
Buffer	Typically in low-Ca²⁺, stabilizing buffer (e.g., with EDTA)
Biological Activity	Functional in standard reconstitution and luminescence assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.