Description

1,24,25-Trihydroxyvitamin D3 is a biologically active metabolite of Vitamin D3, representing a key intermediate in the hormonal calcitriol pathway. This compound is of significant interest for its role in calcium and phosphate metabolism, as well as its potential in cell differentiation and immune modulation research. It is primarily utilized by pharmaceutical R&D laboratories, academic research institutions, and manufacturers of high-purity reference standards and active pharmaceutical ingredients (APIs).

Application

• Pharmaceutical Research & Development: A critical reference standard and intermediate in the synthesis and study of Vitamin D analogs and metabolites for therapeutic applications.
• Biochemical Research: Used to investigate the Vitamin D endocrine system, including receptor binding studies, metabolic pathway analysis, and gene regulation experiments.
• Reference Standard Manufacturing: Serves as a high-purity certified reference material (CRM) for analytical method development, validation, and quality control in pharmaceutical and clinical testing.
• Nutritional Science Studies: Employed in research to understand the complex metabolism and biological activity of Vitamin D3 and its derivatives.
• Cell Culture & In Vitro Studies: Applied in studies examining the effects of Vitamin D metabolites on cell proliferation, differentiation, and function in various cell lines.

Basic Information

Product Name	1,24,25-Trihydroxyvitamin D3
CAS No.	50648-94-7
Molecular Formula	C₂₇H₄₄O₅
Molecular Weight	448.64 g/mol
Synonyms	1,24,25-Trihydroxycholecalciferol; (5Z,7E)-9,10-Secocholesta-5,7,10(19)-triene-1,3,24,25-tetrol; 1α,24,25-Trihydroxyvitamin D3; 1α,24R,25-Trihydroxyvitamin D3; 24,25,26-Trihydroxyvitamin D3; Vitamin D3 metabolite
EINECS	Contact for details

Quality Control

Our 1,24,25-Trihydroxyvitamin D3 is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods (NMR, MS), to ensure it meets the stringent requirements for research and reference standard applications. A detailed Certificate of Analysis (COA) is provided with every shipment, confirming batch-specific results.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. For long-term storage, keep desiccated to maintain stability. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.