Description

3,5-Dinitrophenyl β-D-Galactoside is a specialized chromogenic and fluorogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of β-galactosidase activity, a common reporter enzyme in molecular biology and diagnostic applications. It is primarily utilized by research institutions, diagnostic kit manufacturers, and biotechnology companies for applications in gene expression studies, microbiological testing, and high-throughput screening.

Application

• β-Galactosidase Reporter Assays: A key substrate for detecting lacZ gene expression in molecular biology and genetic engineering.
• Diagnostic Reagents: Used in the formulation of clinical and microbiological diagnostic kits for enzyme-linked detection.
• Biochemical Research: Employed in enzyme kinetics studies and the characterization of β-galactosidase enzymes from various sources.
• Cell-Based Screening: Facilitates high-throughput screening (HTS) in drug discovery for identifying modulators of gene expression.
• Microbiology: Applied in microbial identification and studies of bacterial metabolism, particularly in organisms expressing β-galactosidase.
• Quality Control in Biotechnology: Serves as a reference standard in the quality assurance of recombinant protein production and assay validation.

Basic Information

Product Name	3,5-Dinitrophenyl β-D-Galactoside
CAS No.	50301-19-4
Molecular Formula	C12H14N2O10
Molecular Weight	346.25 g/mol
Synonyms	3,5-Dinitrophenyl β-D-galactopyranoside; DNP-β-Gal; Dinitrophenylgalactoside; β-D-Galactopyranoside, 3,5-dinitrophenyl; 2-(3,5-Dinitrophenoxy)-6-(hydroxymethyl)oxane-3,4,5-triol; Galactopyranoside, 3,5-dinitrophenyl, β-D-; 3,5-Dinitrophenyl-β-D-galactopyranoside
EINECS	Contact for details

Quality Control

Our 3,5-Dinitrophenyl β-D-Galactoside is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and diagnostic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to ISO 9001 quality management standards, and our processes are designed to meet the rigorous requirements of the biotechnology and in-vitro diagnostic (IVD) industries.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, consider desiccant and under inert atmosphere to maintain stability.

Specification

Item	Specification
Appearance	Yellow to light brown crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Enzymatic Activity	Suitable for β-galactosidase assays

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.