Description

NADPH (CAS 53-57-6) is the reduced, active form of nicotinamide adenine dinucleotide phosphate, a critical coenzyme in numerous anabolic biosynthetic pathways. Its primary value lies in its role as a universal electron donor, essential for driving reductive biosynthesis and detoxification reactions. This makes it indispensable for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries, where it is used in enzymatic assays, metabolic studies, and the production of fine chemicals.

Application

• Enzymatic Assays & Diagnostic Kits: Essential cofactor for dehydrogenase-based assays measuring substrates like glucose, lactate, and cholesterol in clinical diagnostics and research.
• Biocatalysis & Pharmaceutical Synthesis: Serves as a coenzyme in cell-free systems and whole-cell biotransformations for the stereoselective synthesis of chiral intermediates and active pharmaceutical ingredients (APIs).
• Cellular Metabolism Research: Used in studies focusing on redox balance, pentose phosphate pathway activity, fatty acid and nucleotide synthesis, and oxidative stress response.
• Antioxidant Systems Study: Critical for regenerating reduced glutathione (GSH) via glutathione reductase, making it vital for research into cellular defense mechanisms.
• Fermentation & Metabolic Engineering: Supports in vivo production of high-value compounds in engineered microbial strains by providing reducing power.
• Reference Standard: Used as a high-purity standard for quality control and calibration in analytical laboratories.

Basic Information

Item	Details
Product Name	NADPH
CAS No.	53-57-6
Molecular Formula	C21H30N7O17P3
Molecular Weight	833.35 g/mol
Synonyms	β-Nicotinamide adenine dinucleotide phosphate, reduced tetrasodium salt; Triphosphopyridine nucleotide, reduced form; TPNH; Coenzyme II reduced; NADP reduced; Dihydrocodehydrogenase II; 2'-(Dihydrogen phosphate) 5'-(trihydrogen pyrophosphate) P'→5'-ester with 1,4-dihydro-1-β-D-ribofuranosylnicotinamide; Reduced Nicotinamide Adenine Dinucleotide Phosphate
EINECS	200-178-1

Quality Control

Our NADPH is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing to ensure high purity and consistency, meeting the stringent requirements for research and industrial applications. A Certificate of Analysis (COA) detailing purity, identification, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). For long-term stability, store in aliquots under an inert atmosphere to prevent oxidation. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to slightly yellow powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95%
Water Content (Karl Fischer)	≤ 5.0 %
Specific Rotation	Contact for details
UV Absorbance Ratio (A260/A340)	≤ 2.5
Biological Activity	Conforms (Enzymatic Assay)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.