Description

Bis(2,5-Dioxopyrrolidin-1-Yl) 2,2'-Disulfanediyldibenzoate is a specialized heterobifunctional crosslinking agent featuring an activated ester and a disulfide bond. This compound is engineered for the selective and reversible conjugation of biomolecules, enabling advanced bioconjugation strategies. It is a critical reagent for researchers and manufacturers in the fields of biopharmaceutical development, proteomics, and diagnostic assay formulation, where controlled protein modification and linker cleavage are required.

Application

• Bioconjugation Reagent: For the site-specific crosslinking of proteins, peptides, and antibodies to other molecules (e.g., drugs, fluorophores, solid supports).
• ADC (Antibody-Drug Conjugate) Linker Development: Serves as a cleavable linker platform, utilizing the reducible disulfide bond for controlled payload release in targeted cancer therapies.
• Protein Immobilization: Used to attach proteins to sensor chips or nanoparticles for biosensor and diagnostic device development.
• Affinity Chromatography Ligand Coupling: For the reversible attachment of ligands to chromatography resins.
• Proteomics Research: As a tool for studying protein-protein interactions and complex formation via crosslinking mass spectrometry (XL-MS).
• Polymer Functionalization: Introducing reactive handles or cleavable sites into synthetic polymers for advanced material science applications.

Basic Information

Product Name	Bis(2,5-Dioxopyrrolidin-1-Yl) 2,2'-Disulfanediyldibenzoate
CAS No.	497262-13-2
Molecular Formula	C22H16N2O8S2
Molecular Weight	500.50 g/mol
Synonyms	1,1'-(Disulfanediylbis(2,1-phenylene))bis(1H-pyrrole-2,5-dione); 2,2'-Dithiodibenzoic Acid Bis(N-succinimidyl) Ester; DSSO Crosslinker; DSSO (Disuccinimidyl sulfoxide analog); Bis(N-succinimidyl) 2,2'-dithiodibenzoate; Heterobifunctional NHS Ester-Disulfide Crosslinker; S-Succinimidyl 2-((2-(2,5-dioxo-2,5-dihydro-1H-pyrrol-1-yl)benzoyl)disulfanyl)benzoate
EINECS	Contact for details

Quality Control

Our Bis(2,5-Dioxopyrrolidin-1-Yl) 2,2'-Disulfanediyldibenzoate is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant quality management standards to support our global customer base in regulated and non-regulated industries.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.