Description

Thymidine (Deoxyribose-13C5; U-15N2) is a stable isotope-labeled nucleoside, specifically designed for advanced analytical and metabolic research. This compound is essential for providing high-fidelity internal standards in mass spectrometry, enabling precise quantification and tracing of DNA synthesis and cellular metabolic pathways. It is a critical reagent for pharmaceutical R&D, academic research, and diagnostic development in fields such as oncology, virology, and metabolomics. The product is supplied with a CAS number of 478511-40-9.

Application

• Mass Spectrometry Internal Standard: Used as a quantitative reference standard in LC-MS/MS and GC-MS for accurate measurement of endogenous thymidine and related metabolites.
• Metabolic Flux Analysis (MFA): Enables the tracing of nucleotide synthesis and salvage pathways in cell culture and in vivo studies using 13C and 15N tracers.
• Pharmaceutical Development: Critical for the pharmacokinetic (PK) and pharmacodynamic (PD) studies of antiviral and anticancer nucleoside analog drugs.
• Diagnostic Assay Development: Serves as a key component in calibrators and controls for clinical diagnostic tests measuring DNA turnover or mitochondrial disorders.
• Biomedical Research: Utilized in studies of cell proliferation, DNA replication, and the mechanism of action of thymidylate synthase inhibitors.
• Nutrient Tracing: Applied in nutritional science to investigate the utilization of nucleosides in gut microbiota and systemic metabolism.

Basic Information

Product Name	Thymidine (Deoxyribose-13C5; U-15N2)
CAS No.	478511-40-9
Molecular Formula	C1013C5H1515N2O5
Molecular Weight	Contact for details
Synonyms	1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil (Deoxyribose-13C5; U-15N2); 2'-Deoxythymidine-13C5,15N2; dT-13C5,15N2; Thymidine-13C5,15N2; 5-Methyl-2'-deoxyuridine-13C5,15N2; Stable Isotope Labeled Thymidine; DNA Nucleoside Standard-13C5,15N2
EINECS	Contact for details

Quality Control

Our stable isotope-labeled products are manufactured under strict quality systems to ensure the highest isotopic and chemical purity for research integrity. Each batch is supported by a comprehensive Certificate of Analysis (COA) detailing isotopic enrichment (Atom % 13C and 15N), chemical purity, and identity confirmation. We adhere to relevant standards for high-purity biochemicals to guarantee reproducible and reliable performance in sensitive analytical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98%
Isotopic Enrichment	≥ 99 atom % 13C; ≥ 98 atom % 15N
Chemical Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.