Description

C12 Nbd L-Threo Dihydro Ceramide (D18:0/12:0) CAS NO 474943-07-2 is a high-purity, fluorescently labeled dihydroceramide analog, a critical intermediate in sphingolipid metabolism research. This compound is specifically engineered for advanced lipidomic studies, enabling precise tracking and quantification of ceramide synthesis and trafficking within cellular systems. It is an essential tool for researchers in pharmaceutical development, biochemistry, and molecular biology investigating lipid signaling pathways, membrane biophysics, and metabolic disorders.

Application

• Biochemical Research: As a key tracer in studying the de novo sphingolipid biosynthesis pathway and the role of dihydroceramide desaturase.
• Cell Biology & Imaging: Fluorescent visualization (NBD label) of intracellular ceramide localization, membrane dynamics, and lipid raft studies.
• Pharmaceutical Screening: A standard substrate for high-throughput screening of inhibitors targeting enzymes in ceramide metabolism for therapeutic development.
• Metabolic Disease Models: Used in research models for diseases like diabetes, neurodegeneration, and cancer where sphingolipid homeostasis is disrupted.
• Lipidomics Standard: Serves as a critical internal standard or reference compound in mass spectrometry-based lipidomic profiling.
• Academic Research: Fundamental research into apoptosis, cell senescence, and stress responses mediated by sphingolipid signaling molecules.

Basic Information

Product Name	C12 Nbd L-Threo Dihydro Ceramide (D18:0/12:0)
CAS No.	474943-07-2
Molecular Formula	C53H91N3O6
Molecular Weight	866.3 g/mol
Synonyms	N-[(1S,2R,3E)-2-Hydroxy-1-(hydroxymethyl)-3-heptadecen-1-yl]-12-[(7-nitro-2-1,3-benzoxadiazol-4-yl)amino]dodecanamide; NBD C12-Dihydroceramide; NBD C12-Dihydro-D-erythro-Ceramide (D18:0/12:0); D-erythro-NBD C12-Dihydroceramide; Fluorescent Dihydroceramide; L-Threo-NBD C12-Dihydroceramide; 2-NBD-C12-Dihydroceramide; NBD-labeled Dihydroceramide
EINECS	Contact for details

Quality Control

Our C12 Nbd L-Threo Dihydro Ceramide is manufactured under strict quality protocols to ensure the highest standards of purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and impurity profiles. We adhere to ISO 9001 quality management principles, and our analytical methods are validated to meet the rigorous demands of the life science research community.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. The product is light-sensitive and easily oxidized. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Solubility	Soluble in DMSO, ethanol, chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.