Description

C12 Nbd-Sphingosine, β-D-Lactosyl is a specialized fluorescently labeled sphingolipid derivative, designed for advanced biochemical and cell biology research. This compound matters as a critical tool for the real-time visualization and tracking of glycosphingolipid metabolism, membrane trafficking, and lipid-protein interactions within live cells. Researchers in pharmaceutical development, academic biochemistry, and diagnostic assay design need this high-purity probe to investigate lipid raft dynamics, cellular signaling pathways, and the role of sphingolipids in disease mechanisms.

Application

• Fluorescent Labeling & Cell Imaging: Acts as a vital fluorescent probe (NBD-labeled) for real-time, non-radioactive tracking of lactosylceramide and related glycosphingolipids in live-cell microscopy and flow cytometry.
• Sphingolipid Metabolism Studies: Used to investigate the biosynthesis, intracellular transport, and catabolic pathways of complex sphingolipids within cellular systems.
• Membrane Biology & Lipid Raft Research: Essential for studying the organization, dynamics, and function of lipid rafts and membrane microdomains where glycosphingolipids are enriched.
• Drug Discovery & Target Validation: Serves as a key reagent in high-content screening assays to identify and validate novel therapeutics targeting sphingolipid-related disorders such as cancer, neurodegeneration, and lysosomal storage diseases.
• Diagnostic Assay Development: Employed in the development of sensitive in vitro diagnostic tests for enzymes involved in sphingolipid metabolism, like glucocerebrosidase or galactocerebrosidase.
• Biochemical Mechanism Elucidation: Facilitates in vitro studies to elucidate enzyme kinetics and inhibitor interactions with specific glycosphingolipid-processing enzymes.

Basic Information

Product Name	C12 Nbd-Sphingosine, β-D-Lactosyl
CAS No.	474943-06-1
Molecular Formula	C38H61N3O13
Molecular Weight	767.91 g/mol
Synonyms	NBD C12-Lactosylceramide; 6-((N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine β-D-lactoside; N-[6-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoyl]-β-D-lactosylsphingosine; Lactosylceramide NBD C12; Fluorescent Lactosylceramide (C12-NBD); D-erythro-N-[6-[(7-Nitro-4-benzofurazanyl)amino]hexanoyl]-1-O-β-D-galactopyranosyl-(1→4)-β-D-glucopyranoside sphingosine; NBD C12-LacCer
EINECS	Contact for details

Quality Control

Every batch of C12 Nbd-Sphingosine, β-D-Lactosyl is manufactured and analyzed under strict quality protocols to ensure the high purity and batch-to-batch consistency required for sensitive research applications. Our quality system includes comprehensive analytical characterization using HPLC, MS, and NMR to confirm identity and purity. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting critical specifications such as assay purity, fluorescence properties, and solvent content.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere. This product is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area Percent)
Solvent Content (Residual Solvents)	Meets ICH guidelines
Fluorescence Properties	Ex/Em ~465/535 nm in methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.