Description

C6 Nbd-Sphingosine, β-D-Lactosyl is a fluorescently labeled sphingolipid derivative, specifically designed for advanced biochemical research. This compound matters as a critical tool for the real-time visualization and tracking of glycosphingolipid metabolism, membrane dynamics, and intracellular trafficking pathways. Researchers in glycobiology, lipidomics, and cell biology need this high-purity probe to investigate complex biological processes with precision.

Application

• Fluorescent Labeling & Imaging: Acts as a vital fluorescent probe (NBD fluorophore) for real-time visualization of glycosphingolipid localization and movement in live or fixed cells.
• Sphingolipid Metabolism Studies: Used to trace the synthesis, degradation, and interconversion pathways of complex sphingolipids within cellular systems.
• Membrane Biology Research: Investigates lipid raft formation, membrane fluidity, and the role of specific glycosphingolipids in cell signaling and membrane organization.
• Intracellular Trafficking Assays: Tracks the transport and sorting of glycosphingolipids through secretory and endocytic pathways.
• Enzyme Activity Assays: Serves as a substrate or inhibitor in assays for glycosyltransferases, glycosidases, and other enzymes involved in sphingolipid metabolism.
• Drug Discovery & Target Validation: Used in high-throughput screening and mechanistic studies for diseases related to sphingolipid imbalances, such as lysosomal storage disorders and cancer.

Basic Information

Product Name	C6 Nbd-Sphingosine, β-D-Lactosyl
CAS No.	474943-04-9
Molecular Formula	C41H63N5O13
Molecular Weight	833.97 g/mol
Synonyms	NBD C6-Lactosylceramide; 6-((N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine β-D-lactoside; NBD C6-LacCer; Lactosylceramide NBD C6; N-[6-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoyl]-D-erythro-sphingosyl-β-D-lactoside; Fluorescent Lactosylceramide C6-NBD; β-D-Lactosyl-N-6-[(7-nitro-2,1,3-benzoxadiazol-4-yl)amino]hexanoyl-D-erythro-sphingosine
EINECS	Contact for details

Quality Control

Our C6 Nbd-Sphingosine, β-D-Lactosyl is produced and handled under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity verification and structural confirmation via mass spectrometry. A detailed Certificate of Analysis (COA) documenting identity, purity, and stability is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This compound is easily oxidized; containers should be sealed under an inert atmosphere (e.g., argon or nitrogen) after each use to maintain purity. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Solubility	Soluble in DMSO, ethanol, chloroform/methanol mixtures

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.