Description

n-[12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoyl]-D-Glucosyl-1-1'-Sphingosine is a sophisticated, fluorescently labeled sphingolipid derivative, specifically designed for advanced cellular and biochemical research. This compound matters as a critical tool for the real-time visualization and tracking of sphingolipid metabolism, membrane trafficking, and glycosphingolipid function within live cells and complex biological systems. Researchers in academic institutions, pharmaceutical R&D, and biotechnology companies require this high-purity probe for studies in lipidomics, cell signaling, and the development of diagnostic assays.

Application

• Fluorescent Labeling of Sphingolipids: Acts as a vital tracer for studying the synthesis, trafficking, and degradation pathways of glycosphingolipids in live-cell imaging.
• Membrane Biology Research: Used to investigate lipid raft dynamics, membrane fluidity, and the role of sphingolipids in cell membrane structure and function.
• Metabolic Pathway Analysis: Enables tracking and quantification of metabolic intermediates in sphingolipid-related pathways for disease mechanism studies.
• Drug Discovery & Target Validation: Serves as a key reagent in high-content screening assays to identify and validate compounds that modulate sphingolipid metabolism, relevant to cancer, neurodegenerative, and metabolic disorders.
• Diagnostic Probe Development: Utilized in the design and development of fluorescent-based diagnostic kits and sensors for detecting enzymatic activities or specific lipid biomarkers.
• Biophysical Studies: Employed in model membrane systems (e.g., liposomes) to study lipid-protein interactions using fluorescence spectroscopy techniques like FRET.

Basic Information

Product Name	n-[12-[(7-Nitro-2-1,3-Benzoxadiazol-4-Yl)Amino]Dodecanoyl]-D-Glucosyl-1-1'-Sphingosine
CAS No.	474943-01-6
Molecular Formula	C₄₂H₆₉N₅O₁₁
Molecular Weight	844.03 g/mol
Synonyms	NBD C12-Glucosylceramide; NBD-GlcCer; 1-O-(β-D-Glucopyranosyl)-N-[(12-[(7-nitro-4-benzofurazanyl)amino]dodecanoyl])-D-erythro-sphingosine; N-[12-[(7-Nitro-2,1,3-benzoxadiazol-4-yl)amino]dodecanoyl]-D-glucosyl-β1-1'-sphingosine; Fluorescent Glucosylceramide; NBD-labeled GlcCer; NBD C12-Glucosylsphingosine
EINECS	Contact for details

Quality Control

This high-value biochemical is manufactured under strict quality control protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Identity and purity are confirmed through a combination of advanced analytical techniques including HPLC, mass spectrometry (MS), and NMR spectroscopy. A comprehensive Certificate of Analysis (COA) detailing purity, identity confirmation, and impurity profile is provided with each shipment to support your research documentation and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store under inert atmosphere. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Solubility	Soluble in DMSO, chloroform, DMF
Fluorescence Properties	Ex/Em ~465/535 nm (in lipid membranes)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.