Description

Ethylenediamine-N,N,N’,N’-Tetraacetic Acid-13C4 is a stable isotope-labeled analog of the essential chelating agent EDTA, where four carbon atoms are replaced with the 13C isotope. This compound is critical for applications requiring precise quantification and tracing of EDTA behavior in complex systems, eliminating spectral interference. It is primarily utilized by researchers and quality control laboratories in the pharmaceutical, environmental, and analytical chemistry sectors who require high-fidelity internal standards for mass spectrometry. The product, identified by CAS No. 470462-56-7, offers unmatched accuracy in method development and validation.

Application

• Internal Standard for Quantitative Analysis: Serves as a crucial reference standard in LC-MS and GC-MS for the accurate quantification of unlabeled EDTA and related chelators in biological and environmental samples.
• Metabolic Tracing Studies: Used in pharmaceutical research to trace the absorption, distribution, metabolism, and excretion (ADME) of EDTA-based compounds and their metal complexes.
• Method Development & Validation: Essential for developing and validating robust analytical methods in compliance with ICH, FDA, and EMA guidelines, ensuring data integrity.
• Environmental Fate Studies: Enables precise tracking of EDTA and its degradation products in water treatment processes and environmental monitoring programs.
• Pharmaceutical Impurity Profiling: Assists in the identification and quantification of EDTA-related impurities in drug substances and finished products.
• Calibration Standard for ICP-MS: Used in elemental analysis to calibrate systems and account for matrix effects when measuring metals chelated by EDTA.

Basic Information

Product Name	Ethylenediamine-N,N,N’,N’-Tetraacetic Acid-13C4
CAS No.	470462-56-7
Molecular Formula	C13C4H16N2O8 or C213C4H16N2O8
Molecular Weight	296.18 g/mol (for 13C4 isotopologue)
Synonyms	(1,2-13C2)Ethylenediaminetetraacetic Acid; EDTA-13C4; 13C4-EDTA; Ethylenedinitrilotetraacetic Acid-13C4; Edetic Acid-13C4; N,N'-1,2-ethanediylbis[N-(carboxymethyl)glycine]-13C4; 13C Labeled EDTA; Isotopic EDTA Internal Standard
EINECS	Contact for details

Quality Control

Every batch of Ethylenediamine-N,N,N’,N’-Tetraacetic Acid-13C4 is manufactured and analyzed under strict quality management systems. We provide comprehensive Certificates of Analysis (COA) detailing isotopic purity (typically ≥99 atom % 13C), chemical purity (by HPLC), and confirmation of structure (by NMR and MS). Our quality protocols are designed to meet the stringent requirements of analytical reference standard applications, ensuring traceability and reliability for your critical research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. This material is hygroscopic (moisture-sensitive). Store in a cool, dry place at a controlled room temperature (15-25°C). For long-term stability, consider storage under an inert atmosphere in a desiccator.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥98.0%
Isotopic Purity (13C)	≥99 atom %
Isotopic Enrichment (Positional)	≥98% 13C at designated positions
Water Content (KF)	≤2.0%
Residue on Ignition	≤0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.