Description

15-Biotinlamino-4,7,10,13-Dioxanonanoic Acid n-Hydroxysuccinimidyl Ester is a high-purity, heterobifunctional crosslinking reagent designed for advanced bioconjugation. This compound is critical for researchers and manufacturers requiring precise, stable labeling of biomolecules with biotin for detection, purification, and diagnostic applications. It is primarily utilized in the development of sensitive immunoassays, affinity chromatography media, and targeted drug delivery systems within the pharmaceutical, diagnostic, and life science research sectors.

Application

• Immunoassay Development: Conjugation of biotin to antibodies, antigens, or other proteins for use in ELISA, lateral flow assays, and other diagnostic platforms.
• Affinity Chromatography: Synthesis of biotinylated ligands for immobilization on streptavidin/avidin resins to purify target proteins or nucleic acids.
• Cell Surface Labeling: Biotinylation of cell surface proteins or receptors for flow cytometry, fluorescence microscopy, or cell sorting applications.
• Drug Conjugate Synthesis: Creation of biotin-drug or biotin-polymer conjugates for targeted therapeutic and diagnostic (theranostic) applications.
• Microarray & Biosensor Fabrication: Immobilization of biotinylated capture probes on sensor surfaces to enhance specificity and signal detection.
• Protein-Protein Interaction Studies: Crosslinking agents for studying biomolecular interactions via pull-down assays or mass spectrometry.
• Nanoparticle Functionalization: Attachment of biotin to nanoparticles for creating targeted delivery systems or diagnostic imaging agents.
• Oligonucleotide Labeling: Biotinylation of DNA or RNA probes for in situ hybridization, sequencing, and molecular diagnostics.

Basic Information

Product Name	15-Biotinlamino-4,7,10,13-Dioxanonanoic Acid n-Hydroxysuccinimidyl Ester
CAS No.	459426-22-3
Molecular Formula	C23H38N4O8S
Molecular Weight	530.63 g/mol
Synonyms	Biotin-PEG4-NHS Ester; Biotinamidohexanoyl-PEG4-NHS; Biotin-PEG4-NHS; Biotin-(PEG)4-NHS ester; NHS-PEG4-Biotin; 15-(Biotinylamino)-4,7,10,13-tetraoxapentadecanoic acid N-succinimidyl ester; Biotinylated PEG NHS ester (PEG4 spacer); EZ-Link Biotin-PEG4-NHS Ester (common trade name)
EINECS	Contact for details

Quality Control

Our 15-Biotinlamino-4,7,10,13-Dioxanonanoic Acid n-Hydroxysuccinimidyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity testing to ensure optimal performance in conjugation reactions. Certificates of Analysis (COA) detailing purity, identity, and specific impurities are provided with every shipment to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M+H]+
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile
Functional Activity	≥ 90% active ester by amine coupling test

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.