Description

Methotrexate-D3 is a deuterium-labeled analog of the widely used chemotherapeutic and immunosuppressive agent methotrexate. This stable isotope-labeled compound is essential for use as an internal standard in quantitative mass spectrometry, ensuring the highest accuracy and reliability in analytical measurements. It is a critical tool for pharmaceutical research, clinical diagnostics, and bioanalytical laboratories requiring precise pharmacokinetic and metabolic studies.

Application

• Internal Standard for LC-MS/MS: Primary application as a quantitative reference standard in liquid chromatography-tandem mass spectrometry for bioanalysis.
• Pharmacokinetic Studies: Enables accurate measurement of methotrexate concentration in plasma, serum, and other biological matrices.
• Therapeutic Drug Monitoring (TDM): Supports clinical assays to optimize methotrexate dosing and manage toxicity in patients.
• Drug Metabolism Research: Used in ADME (Absorption, Distribution, Metabolism, Excretion) studies to track the parent drug and metabolites.
• Method Development & Validation: Critical for developing and validating robust, regulatory-compliant analytical methods in GLP/GMP environments.
• Forensic & Toxicology Analysis: Provides definitive quantification in forensic investigations and toxicological screenings.

Basic Information

Product Name	Methotrexate-D3
CAS No.	432545-63-6
Molecular Formula	C₂₀H₂₀D₃N₈O₅
Molecular Weight	458.44 g/mol
Synonyms	(2S)-2-[[4-[(2,4-Diaminopteridin-6-yl)methyl-methylamino]benzoyl]amino]pentanedioic-3,3,4,4-d4 acid; Deuterated Methotrexate; Methotrexate-d3; MTX-d3; 4-Amino-10-methylfolic acid-d3; Amethopterin-d3; L-Glutamic acid, N-[4-[[(2,4-diamino-6-pteridinyl)methyl]methylamino]benzoyl]-, 3,3,4,4-d4
EINECS	Contact for details

Quality Control

Our Methotrexate-D3 is manufactured under strict quality systems to ensure the high chemical and isotopic purity required for analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and MS, to confirm identity, purity, and deuterium incorporation. A comprehensive Certificate of Analysis (COA) detailing isotopic purity, chemical purity, and analytical results is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and should be kept in a dry environment. For long-term storage, consider desiccant use and inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (deuterium incorporation)	≥ 98 atom % D
Chemical Purity	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.