Description

5-Bromo-6-Chloro-3-Indoxyl Phosphate, Disodium Salt Trihydrate is a high-purity chromogenic substrate essential for enzymatic detection systems. This compound is critical for researchers and diagnostic manufacturers requiring precise, sensitive, and reproducible colorimetric assays. It is primarily used in molecular biology, histochemistry, and diagnostic kit development for applications such as alkaline phosphatase (AP) and other phosphatase-linked detection.

Application

• Enzymatic Substrate for Alkaline Phosphatase (AP): A key chromogenic agent in ELISA, Western blotting, and immunohistochemistry (IHC) for generating an insoluble blue indigo dye precipitate.
• Molecular Biology Research: Used in nucleic acid hybridization techniques, including Southern, Northern, and colony blot assays, for non-radioactive detection.
• Diagnostic Kit Manufacturing: A critical raw material in the production of clinical and point-of-care diagnostic tests for various biomarkers.
• Histology and Cytology: Enables precise localization of enzyme activity in tissue sections and cell cultures for pathological and research studies.
• Microbiology: Employed in microbial detection and identification systems that utilize phosphatase activity as an indicator.
• Biochemical Assay Development: Serves as a standard reagent for developing and validating new quantitative and qualitative phosphatase activity assays.

Basic Information

Item	Details
Product Name	5-Bromo-6-Chloro-3-Indoxyl Phosphate, Disodium Salt Trihydrate
CAS No.	404366-59-2
Molecular Formula	C8H5BrClNO4P · 2Na · 3H2O
Molecular Weight	Contact for details
Synonyms	5-Bromo-6-chloro-3-indolyl phosphate disodium salt; BCIP, Disodium Salt; 5-Bromo-4-chloro-3-indolyl phosphate disodium salt (common misnomer); X-Phosphate; BCIP Disodium Salt Trihydrate; 5-Br-6-Cl-3-Indoxyl Phosphate Disodium Salt; Phosphoric acid (5-bromo-6-chloro-1H-indol-3-yl) ester disodium salt trihydrate
EINECS	Contact for details

Quality Control

Every batch of 5-Bromo-6-Chloro-3-Indoxyl Phosphate, Disodium Salt Trihydrate is manufactured and tested under a strict quality management system. We provide comprehensive Certificates of Analysis (COA) that detail purity, identity, and performance in standard assay systems. Our quality protocols are designed to meet the stringent requirements of diagnostic and research-grade chemical production, ensuring batch-to-batch consistency and reliability for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability and performance. For long-term storage, consider desiccant in the storage environment.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	8.0% - 12.0%
Solubility	Soluble in water, clear solution
Performance in AP Assay	Produces characteristic blue precipitate

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.