Description

3X Flag Peptide CAS NO 402750-12-3 is a high-purity synthetic peptide sequence incorporating a triple Flag tag, a widely recognized epitope tag in molecular biology and protein research. This compound is critical for the affinity purification, detection, and functional analysis of recombinant fusion proteins. It is an essential reagent for researchers and manufacturers in the biotechnology and pharmaceutical sectors, particularly those involved in proteomics, antibody development, and high-throughput screening platforms.

Application

• Affinity Purification: Used as a capture ligand immobilized on resin for the purification of Flag-tagged recombinant proteins via immunoaffinity chromatography.
• Protein Detection & Western Blotting: Serves as a standard or competitor in assays utilizing anti-Flag antibodies for the specific detection and quantification of tagged proteins.
• Biomolecular Interaction Studies: Employed in surface plasmon resonance (SPR) or ELISA to study protein-protein or protein-antibody interactions involving the Flag epitope.
• Cell Biology Research: Utilized in pull-down assays and immunoprecipitation experiments to isolate and characterize protein complexes from cell lysates.
• Diagnostic Kit Development: Acts as a key component in the development and calibration of diagnostic kits and assays targeting Flag-tagged analytes.
• Pharmaceutical R&D: Applied in drug discovery pipelines for target validation and the study of therapeutic proteins and antibodies.

Basic Information

Product Name	3X Flag Peptide
CAS No.	402750-12-3
Molecular Formula	C82H134N26O27S
Molecular Weight	1916.1 g/mol
Synonyms	Triple Flag Peptide; 3xFLAG Peptide; DYKDHDGDYKDHDIDYKDDDDK Peptide; FLAG Tag Peptide (3x); Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys; Anti-FLAG M2 Affinity Gel Elution Peptide; TFA salt of 3X-FLAG peptide
EINECS	Contact for details

Quality Control

Every batch of our 3X Flag Peptide is manufactured under controlled conditions and subjected to rigorous analytical testing to ensure identity, purity, and consistency. We adhere to high standards suitable for research and bioprocessing applications. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), molecular weight confirmation (by MS), and endotoxin levels is provided with each shipment to guarantee traceability and quality assurance.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC & MS)	Conforms to standard
Purity (HPLC)	≥ 95%
Molecular Weight	1916.1 ± 2 Da
Amino Acid Composition	Conforms to theoretical values
Water Content (KF)	≤ 8.0%
Endotoxin	< 10 EU/mg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.