Description

Mca-Leu-Glu-Val-Asp-Gly-Trp-Lys(Dnp)-Nh2 is a specialized, sequence-specific fluorogenic peptide substrate designed for the study of caspase and other protease activities. This compound matters for its high sensitivity and selectivity in enzymatic assays, enabling precise measurement of protease kinetics and inhibition in biochemical research. It is primarily needed by pharmaceutical R&D teams, academic biochemistry laboratories, and diagnostic kit manufacturers focused on apoptosis research, drug discovery, and enzyme mechanism studies.

Application

• Biochemical Research: As a fluorogenic substrate for studying the activity and kinetics of caspases, particularly caspase-3 and related proteases involved in apoptosis.
• Drug Discovery & Screening: Used in high-throughput screening (HTS) assays to identify and characterize novel protease inhibitors for therapeutic development.
• Apoptosis Pathway Analysis: A critical tool for quantifying programmed cell death in cell-based assays and understanding disease mechanisms in cancer and neurodegenerative disorders.
• Diagnostic Development: Employed in the development of clinical and research diagnostic kits for measuring specific protease activity in biological samples.
• Enzyme Characterization: For determining the substrate specificity, catalytic efficiency (kcat/Km), and inhibition constants (Ki) of various proteolytic enzymes.
• Academic & Teaching Laboratories: Used in university settings for advanced biochemistry and molecular biology practical courses.

Basic Information

Product Name	Mca-Leu-Glu-Val-Asp-Gly-Trp-Lys(Dnp)-Nh2
CAS No.	400727-81-3
Molecular Formula	C58H74N14O19
Molecular Weight	1247.3 g/mol
Synonyms	Ac-DEVD-AFC; Caspase-3 Substrate I (Ac-DEVD-AFC); (7-Methoxycoumarin-4-yl)acetyl-Leu-Glu-Val-Asp-Gly-Trp-Lys(2,4-dinitrophenyl)-NH2; Mca-L-E-D-V-D-G-W-K(Dnp)-NH2; Fluorogenic Caspase-3 Substrate; Ac-Asp-Glu-Val-Asp-7-amido-4-trifluoromethylcoumarin (related analog); Ac-DEVD-MCA (common naming convention); AFC Caspase Substrate
EINECS	Contact for details

Quality Control

Our Mca-Leu-Glu-Val-Asp-Gly-Trp-Lys(Dnp)-Nh2 is produced under stringent conditions to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes rigorous quality testing, including purity verification by HPLC and identity confirmation by mass spectrometry, to ensure compliance with research-grade standards. Certificates of Analysis (COA) detailing purity, identity, and performance data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake. For long-term storage, keep desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; Slightly soluble in water
Functional Activity	Conforms to reference standard in caspase-3 assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.