Description

Ac-Glu-Asp(Edans)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(Dabcyl)-Glu-Nh2 CAS NO 400716-78-1 is a specialized, high-purity synthetic peptide substrate designed for advanced biochemical research and diagnostic applications. This compound is engineered as a fluorescent resonance energy transfer (FRET) substrate, making it an essential tool for the sensitive detection and quantification of specific protease activity. It is primarily utilized by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions engaged in enzymology, biomarker discovery, and high-throughput screening.

Application

• Protease Activity Assays: As a FRET-based substrate for the specific detection and kinetic analysis of target proteases in biochemical research.
• Drug Discovery Screening: High-throughput screening (HTS) of compound libraries for inhibitors or activators of specific proteolytic enzymes.
• Diagnostic Development: A key component in the development of in vitro diagnostic (IVD) assays for clinical biomarkers related to protease activity.
• Enzyme Mechanism Studies: Investigating the catalytic mechanism, specificity, and inhibition profiles of proteases.
• Cell Biology Research: Monitoring intracellular protease activity in live-cell imaging and signaling pathway analysis.
• Quality Control in Biologics: Testing for the presence and activity of specific proteases during biopharmaceutical manufacturing processes.

Basic Information

Product Name	Ac-Glu-Asp(Edans)-Lys-Pro-Ile-Leu-Phe-Phe-Arg-Leu-Gly-Lys(Dabcyl)-Glu-Nh2
CAS No.	400716-78-1
Molecular Formula	C₁₀₀H₁₄₈N₂₈O₂₈S₂
Molecular Weight	2247.6 g/mol
Synonyms	FRET Substrate for Protease; Edans/Dabcyl FRET Peptide; Acetyl-Glutamic acid-Aspartic acid(Edans)-Lysine-Proline-Isoleucine-Leucine-Phenylalanine-Phenylalanine-Arginine-Leucine-Glycine-Lysine(Dabcyl)-Glutamic acid amide; Protease Detection Substrate; Fluorescent Peptide Substrate; Dabcyl-Edans FRET Pair Peptide; Intramolecularly Quenched Fluorogenic Peptide; Custom Protease Substrate 400716-78-1
EINECS	Contact for details

Quality Control

Every batch of this high-purity peptide is manufactured under strict quality systems and undergoes comprehensive analytical testing. We guarantee identity, purity, and performance through multiple orthogonal methods including HPLC, MS, and amino acid analysis. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity (typically >95% by HPLC), peptide content, and other critical specifications to ensure batch-to-batch consistency for your research and development needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot into single-use quantities to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by weight)
Amino Acid Analysis	Within ± 10% of theoretical
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; Aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.