Description

2-Thio-Fad is a specialized biochemical compound, a thio-analogue of Flavin Adenine Dinucleotide (FAD), with the CAS registry number 43203-75-4. This modified coenzyme is valued for its role in probing and modulating the activity of flavin-dependent enzymes, which are critical in numerous metabolic and redox processes. It is an essential research tool for biochemists, enzymologists, and pharmaceutical developers working on metabolic pathways, enzyme mechanisms, and drug discovery.

Application

• Enzyme Mechanism Studies: Used as a substrate analogue to investigate the catalytic mechanisms and binding sites of flavoproteins and flavoenzymes.
• Biochemical Research: Serves as a critical reagent in academic and industrial R&D for studying electron transfer, oxidation-reduction reactions, and metabolic engineering.
• Pharmaceutical Development: Employed in high-throughput screening and inhibitor design for diseases involving flavin-dependent enzymes, such as certain cancers and microbial infections.
• Diagnostic Assays: Can be utilized in the development of diagnostic kits and biosensors that rely on specific flavoenzyme activity.
• Metabolic Pathway Analysis: Acts as a tool to dissect and understand complex metabolic networks in cell biology and systems biology.

Basic Information

Product Name	2-Thio-Fad
CAS No.	43203-75-4
Molecular Formula	C27H33N9O15P2S
Molecular Weight	849.62 g/mol
Synonyms	2-Thio-FAD; 2-Thioflavin Adenine Dinucleotide; Thio-FAD; 2-Mercapto-FAD; FAD 2-Thioanalogue; Adenosine 5'-(trihydrogen diphosphate), P'→5'-ester with 2-thio-1-deoxy-1-(7,8-dimethyl-2,4-dioxo-2H-benzo[g]pteridin-10(4H)-yl)-D-ribitol; 2-Thioriboflavin adenine dinucleotide
EINECS	Contact for details

Quality Control

Our 2-Thio-Fad is produced under stringent quality management protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards for research-grade biochemicals to guarantee reliable performance in your experiments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. For long-term storage, keep desiccated under an inert atmosphere. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange lyophilized powder
Identification (HPLC/MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 3.0%
Solubility	Soluble in water and buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.