Description

Cytohelicase CAS NO 42613-29-6 is a specialized enzyme preparation widely recognized for its cell wall degrading properties. This product is critical for applications requiring the gentle and efficient release of protoplasts from plant, fungal, or microbial cells. It is an essential research and production tool for scientists and technicians in life sciences and biotechnology. Key industries that rely on this enzyme include pharmaceutical development, agricultural biotechnology, and fundamental biochemical research.

Application

• Protoplast Isolation: Primary application for generating plant, fungal, and microbial protoplasts for genetic studies and transformation.
• Cell Biology Research: Used to study cell wall composition, structure, and the dynamics of cell membrane function in a controlled manner.
• Enzymatic Digestion in Bioprocessing: Facilitates the breakdown of complex cellular matrices in downstream processing for metabolite extraction.
• Plant Tissue Culture: Aids in the preparation of single cells or small cell aggregates from callus or plant tissues for suspension culture initiation.
• Fungal Genetics: Enables the creation of fungal spheroplasts for molecular biology techniques such as DNA transformation and fusion.
• Biocatalyst and Enzyme Studies: Serves as a model enzyme for studying hydrolytic activity on specific polysaccharide bonds.

Basic Information

Product Name	Cytohelicase
CAS No.	42613-29-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cellulase (from Helix pomatia); Cytophagic Enzyme; Cytophagic Factor; Helix Pomatia Cellulase; Snail Gut Enzyme; Cell Wall Degrading Enzyme; Protoplast Release Enzyme; β-Glucanase (partial activity)
EINECS	Contact for details

Quality Control

Our Cytohelicase is produced and tested under strict quality management protocols to ensure batch-to-batch consistency and optimal enzymatic activity. Each lot is characterized for specific activity units and screened for contaminating enzymatic activities. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and stability is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C. This material is hygroscopic (moisture-sensitive) and must be kept in a dry environment. For long-term stability, consider storage at -20°C under desiccant.

Specification

Item	Specification
Appearance	Lyophilized powder or buffered solution
Identification (Activity)	Conforms
Specific Activity	> 1.0 U/mg protein (or as per customer grade)
Protein Content (Biuret)	Reported on COA
pH (in solution)	4.5 - 6.5
Endotoxin Level	< 10 EU/mg (for research grades)
Microbial Limits	Conforms to in-house specifications

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.