Description

Diethyl N,n-Diisopropylphosphoramidite (CAS NO 42053-26-9) is a highly reactive organophosphorus compound primarily used as a key phosphitylating agent in oligonucleotide synthesis. Its core value lies in enabling the efficient and controlled formation of internucleotide phosphate linkages, which is fundamental for producing DNA and RNA strands. This chemical is essential for manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors who are developing nucleic acid-based therapeutics, primers, and probes.

Application

• Phosphoramidite Chemistry in Oligonucleotide Synthesis: Serves as a critical reagent in solid-phase synthesis for the automated production of DNA and RNA sequences.
• Pharmaceutical Intermediate: Used in the research and development of antisense oligonucleotides, siRNA, and other nucleic acid therapeutics.
• Diagnostic Reagent Manufacturing: Employed in the production of labeled probes and primers for PCR and genetic testing kits.
• Academic and Industrial Research: A vital tool in chemical biology and biochemistry labs for studying nucleic acid structure and function.
• Precursor for Modified Nucleotides: Acts as a building block for synthesizing nucleoside phosphoramidites with specialized protective groups or labels.
• Ligand Synthesis in Catalysis: Can be utilized as an intermediate for preparing chiral phosphine ligands used in asymmetric catalysis.

Basic Information

Product Name	Diethyl N,n-Diisopropylphosphoramidite
CAS No.	42053-26-9
Molecular Formula	C10H24NO2P
Molecular Weight	221.28 g/mol
Synonyms	Diethyl Diisopropylphosphoramidite; Phosphoramidous acid, diisopropyl-, diethyl ester; Diethyl N,N-diisopropylphosphoramidite; Diisopropylphosphoramidous Acid Diethyl Ester; Diethyl (Diisopropylamino)phosphonite; (Diisopropylamino)diethoxyphosphine; Amidite Coupling Reagent
EINECS	Contact for details

Quality Control

Our Diethyl N,n-Diisopropylphosphoramidite is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, critical for sensitive oligonucleotide synthesis. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and moisture content. We adhere to cGMP principles where applicable, and our quality protocols are designed to meet the stringent requirements of pharmaceutical and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. Due to its highly moisture-sensitive and easily oxidized nature, this product must be stored under an inert atmosphere (argon or nitrogen) in a cool, dry place. Recommended storage temperature is 2-8°C for long-term stability. Allow the sealed container to reach room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Colorless to pale yellow clear liquid
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.1%
31P NMR Purity	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.