Description

1-Naphthyl-β-D-Galactopyranoside is a specialized chromogenic and fluorogenic substrate used in enzymatic assays. This compound is critical for the detection and quantification of β-galactosidase activity, a widely used reporter system in molecular biology and diagnostic applications. It is primarily utilized by researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic reagent industries for applications ranging from gene expression studies to microbial detection.

Application

• Enzymatic Reporter Assays: As a key substrate for detecting β-galactosidase (lacZ) activity in molecular biology, used in gene expression studies, promoter analysis, and transfection efficiency monitoring.
• Diagnostic Reagents: Employed in the formulation of clinical and research diagnostic kits for the detection of specific enzymes or microorganisms, such as E. coli, in water and food safety testing.
• Histochemistry & Cell Biology: Used in staining techniques (X-gal staining) to visualize spatial gene expression patterns in tissues, embryos, and cultured cells.
• High-Throughput Screening (HTS): Serves as a substrate in drug discovery platforms to identify modulators of gene expression or enzyme activity.
• Biochemical Research: For the study of enzyme kinetics, inhibition, and the characterization of novel β-galactosidase enzymes.
• Microbiology: Incorporated into selective agar media for the identification and differentiation of bacterial colonies based on β-galactosidase production.

Basic Information

Item	Detail
Product Name	1-Naphthyl-β-D-Galactopyranoside
CAS No.	41335-32-4
Molecular Formula	C16H18O6
Molecular Weight	306.31 g/mol
Synonyms	1-Naphthyl β-D-galactopyranoside; 1-Naphthyl-β-D-galactoside; β-D-Galactopyranoside, 1-naphthyl; 6-(1-Naphthyl)-β-D-galactopyranoside; 4-Nitrophenyl-β-D-galactopyranoside (common analog); ONPG (common analog name context); β-Galactosidase substrate; Chromogenic galactoside substrate.
EINECS	Contact for details

Quality Control

Our 1-Naphthyl-β-D-Galactopyranoside is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Each lot is supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and performance in standard enzymatic assays. We adhere to relevant quality guidelines to support our customers in regulated environments.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. This material is light-sensitive (store away from light).

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Performance (Enzymatic Assay)	Suitable for β-galactosidase detection

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.