Description

L-Arginine p-Nitroanilide Dihydrochloride is a specialized chromogenic substrate widely used in enzymatic assays and biochemical research. This compound is critical for the sensitive and specific detection of trypsin-like proteases, including thrombin and plasmin, due to its release of p-nitroaniline upon cleavage. It is an essential reagent for pharmaceutical R&D, diagnostic kit manufacturing, and academic research in fields like hematology and cell biology. The product is supplied as a high-purity, ready-to-use substrate for reliable and reproducible experimental results.

Application

• Protease Activity Assays: Primary substrate for the spectrophotometric determination of trypsin, thrombin, and plasmin activity in research and quality control.
• Diagnostic Reagent Manufacturing: A key component in the production of in-vitro diagnostic (IVD) kits for coagulation disorders and other clinical assays.
• Pharmaceutical Research & Development: Used in high-throughput screening (HTS) to identify and characterize novel protease inhibitors for drug discovery.
• Biochemical Research: Essential tool for studying enzyme kinetics, inhibition mechanisms, and protease function in academic and industrial laboratories.
• Quality Control in Bioprocessing: Monitors the activity and purity of enzyme preparations used in biomanufacturing processes.

Basic Information

Product Name	L-Arginine p-Nitroanilide Dihydrochloride
CAS No.	40127-11-5
Molecular Formula	C12H20Cl2N6O3
Molecular Weight	367.24 g/mol
Synonyms	L-Arginine 4-nitroanilide dihydrochloride; Arg-pNA•2HCl; BAPNA•2HCl (Benzoyl-L-arginine p-nitroanilide hydrochloride); Nα-Benzoyl-L-arginine 4-nitroanilide hydrochloride; Tosyl-Gly-Pro-Arg-p-nitroanilide acetate salt substrate analog; Protease chromogenic substrate; Thrombin substrate; Trypsin substrate; Chromozym TH
EINECS	Contact for details

Quality Control

Our L-Arginine p-Nitroanilide Dihydrochloride is manufactured under strict quality systems to ensure batch-to-batch consistency for critical research and diagnostic applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectrophotometric verification of functional activity. Certificates of Analysis (COA) detailing identity, purity, assay, and related substances are provided to guarantee it meets the stringent requirements for biochemical and diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of moisture. For long-term storage, consider desiccant.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Functional Activity	Conforms (Spectrophotometric Assay)
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.