Description

Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-Nh2 is a synthetic, modified peptide sequence designed for advanced biochemical research and pharmaceutical development. This compound is valued for its specific structure, which incorporates the chromophore 2,4-dinitrophenyl (Dnp) on a diaminopropionic acid (Dap) residue, making it a critical tool for studying enzyme-substrate interactions, particularly for proteases like dipeptidyl peptidase IV (DPP-IV). It serves as a specialized substrate or inhibitor in proteomics research, drug discovery programs, and diagnostic assay development. This peptide is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors requiring high-purity, well-characterized biochemical tools.

Application

• Protease Activity Assays: Used as a chromogenic or fluorogenic substrate for kinetic studies and inhibition profiling of enzymes like DPP-IV and related proline-specific proteases.
• Drug Discovery & Screening: Serves as a key biochemical tool in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for therapeutic targets.
• Biochemical Research: Employed in academic and industrial laboratories to investigate enzyme mechanisms, specificity, and protein-protein interactions.
• Diagnostic Reagent Development: Acts as a core component in the development of in-vitro diagnostic (IVD) kits for measuring specific protease activities in clinical samples.
• Peptide & Medicinal Chemistry: Used as an intermediate or reference standard in the synthesis and quality control of more complex peptide-based pharmaceuticals.

Basic Information

Product Name	Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-Nh2
CAS No.	396717-35-4
Molecular Formula	C58H86N16O17
Molecular Weight	1263.4 g/mol
Synonyms	7-Methoxycoumarin-4-acetyl-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-amide; Mca-PKPLAL-Dap(Dnp)-AR-NH2; DPP-IV Substrate (Mca-modified); Fluorogenic DPP-IV Substrate; Dap(Dnp)-containing Peptide; Pro-Leu-Ala-Leu Peptide Derivative; Chromogenic Peptide Substrate; (2,4-Dinitrophenyl)-L-2,3-diaminopropionyl Peptide
EINECS	Contact for details

Quality Control

Our Mca-Pro-Lys-Pro-Leu-Ala-Leu-Dap(Dnp)-Ala-Arg-Nh2 is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification, mass spectrometry (MS) for identity confirmation, and amino acid analysis (AAA). We provide Certificates of Analysis (COA) detailing purity, peptide content, and solvent residues. Production can adhere to GMP guidelines for applications requiring the highest level of traceability and documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Peptide Content	≥ 80% (by AAA)
Water Content (KF)	≤ 5.0%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.