Description

H-Thr-Thr-Pna is a specialized peptide derivative containing a p-nitroanilide (Pna) chromogenic group. This compound is primarily valued as a high-purity biochemical substrate for enzymatic activity studies and protease research. It is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors who require precise tools for enzyme kinetics, inhibitor screening, and diagnostic assay development.

Application

• Protease Substrate: Used as a chromogenic substrate for the kinetic analysis and characterization of various serine and threonine proteases.
• Enzyme Inhibitor Screening: Employed in high-throughput screening (HTS) assays to identify and evaluate potential protease inhibitors for drug discovery.
• Biochemical Research: Serves as a critical reagent in fundamental research to study enzyme mechanisms, specificity, and catalytic activity.
• Diagnostic Kit Development: Utilized in the development of colorimetric diagnostic assays and kits for clinical and research laboratories.
• Pharmaceutical R&D: Applied in the research and development stages of therapeutic agents targeting proteolytic pathways.
• Quality Control Testing: Acts as a reference standard in quality control processes for enzyme production and purification.

Basic Information

Product Name	H-Thr-Thr-Pna
CAS No.	390361-42-9
Molecular Formula	C17H25N5O7
Molecular Weight	411.42 g/mol
Synonyms	H-Threonyl-Threonine p-Nitroanilide; Thr-Thr-pNA; L-Threonyl-L-threonine 4-nitroanilide; H-Thr-Thr-pNA; p-Nitroanilide of H-Thr-Thr-OH; Substrate for Protease; Chromogenic Peptide Substrate; 390361-42-9
EINECS	Contact for details

Quality Control

Our H-Thr-Thr-Pna is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and specific tests for identity and related substances. Certificates of Analysis (COA) detailing all specifications are provided to ensure traceability and compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Single Impurity (HPLC)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.