Description

1,2-Distearoyl-Sn-Glycero-3-Phosphoethanolamine-n-[Biotinyl(Polyethylene Glycol)-2000] (Ammonium Salt) is a high-purity, functionalized phospholipid-PEG-biotin conjugate designed for advanced bioconjugation and surface modification. This compound is critical for creating stable, targeted delivery systems and biosensing platforms due to its unique combination of lipid anchoring, PEG spacer for stealth properties, and biotin for high-affinity streptavidin binding. It is an essential material for researchers and formulators in the pharmaceutical, diagnostic, and life science sectors developing next-generation therapeutics and analytical assays.

Application

• Liposome and Lipid Nanoparticle (LNP) Functionalization: For creating targeted drug delivery vehicles, such as those used in mRNA vaccines and cancer therapeutics, by incorporating biotin ligands for subsequent attachment of targeting moieties.
• Biosensor and Diagnostic Assay Development: As a key component for immobilizing biomolecules (e.g., antibodies, DNA) onto sensor surfaces via biotin-streptavidin chemistry, enhancing sensitivity and specificity.
• Cell Membrane Engineering and Labeling: To incorporate biotin groups into cell membranes for studies involving cell sorting, tracking, or the assembly of artificial cell surfaces.
• Protein and Antibody Conjugation: Enables the site-specific biotinylation of proteins through amine-reactive chemistry, crucial for ELISA, western blotting, and pull-down assays.
• Micelle and Polymer Stabilization: Used in the synthesis of functionalized micelles for solubilizing hydrophobic drugs and creating stealth carriers with extended circulation times.
• Surface Plasmon Resonance (SPR) and Quartz Crystal Microbalance (QCM): For constructing robust, non-fouling sensor chips with high ligand loading capacity.
• Affinity Chromatography Media: As a functional ligand for preparing streptavidin-coated resins used in the purification of biotinylated biomolecules.
• Nanomaterial Coating: To impart biocompatibility and functional handles to nanoparticles (e.g., gold, iron oxide) for biomedical imaging and therapeutic applications.

Basic Information

Product Name	1,2-Distearoyl-Sn-Glycero-3-Phosphoethanolamine-n-[Biotinyl(Polyethylene Glycol)-2000] (Ammonium Salt)
CAS No.	385437-57-0
Molecular Formula	C~117H~229N4O~55P (Average, PEG MW ~2000)
Molecular Weight	~2800 g/mol (Average)
Synonyms	DSPE-PEG(2000)-Biotin; 1,2-Distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000]; DSPE-PEG2k-Biotin; Biotinyl-Cap-DSPE-PEG; Biotin-DSPE-PEG(2000); Phospholipid-PEG-Biotin; DSPE-PEG-Biotin, MW 2000; 18:0 PEG2000 Biotin
EINECS	Contact for details

Quality Control

Every batch of our DSPE-PEG(2000)-Biotin is manufactured under strict quality management systems and undergoes comprehensive analytical characterization to ensure identity, purity, and functional performance. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes data from NMR, HPLC, and MS analysis. Our production adheres to cGMP guidelines where applicable, supporting its use in preclinical and diagnostic development. Specifications and COAs are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to prevent condensation. For long-term storage, keep under an inert atmosphere (argon or nitrogen).

Specification

Item	Specification
Appearance	White to off-white solid or waxy solid
Identification (NMR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 95%
Molecular Weight (MS)	~2800 ± 50 Da (Average)
Biotin Content	≥ 0.9 mol biotin / mol phospholipid
Residual Solvents	Meets ICH guidelines
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.