Description

Histone H3 (1-20) is a synthetic peptide fragment corresponding to the N-terminal residues 1 to 20 of the human Histone H3 protein. This sequence is a critical substrate for studying post-translational modifications, such as methylation and acetylation, which regulate gene expression and chromatin dynamics. It is an essential research tool for biochemists, molecular biologists, and pharmaceutical researchers working in epigenetics, oncology, and drug discovery.

Application

• Epigenetic Research: Primary substrate for investigating histone-modifying enzymes like methyltransferases (HMTs) and acetyltransferases (HATs).
• Biochemical Assay Development: Used in activity assays, inhibitor screening, and kinetic studies for drug discovery targeting epigenetic pathways.
• Antibody Production & Validation: Acts as an immunogen or antigen for generating and testing antibodies specific to modified or unmodified Histone H3.
• Chromatin Structure Studies: Essential for in vitro reconstitution experiments to understand nucleosome assembly and chromatin remodeling.
• Diagnostic Tool Development: Potential use in developing assays for detecting epigenetic biomarkers associated with various diseases.
• Academic & Pharmaceutical R&D: Fundamental reagent for basic research and high-throughput screening in oncology and neurology.

Basic Information

Product Name	Histone H3 (1-20)
CAS No.	380598-30-1
Molecular Formula	C101H159N41O26
Molecular Weight	2270.6 g/mol
Synonyms	H3 (1-20) Peptide; Histone H3 Peptide (1-20); ARTKQTARKSTGGKAPRKQLA; Acetyl-Histone H3 (1-20); H3K4me0 (1-20); H3K9me0 (1-20); Human Histone H3 N-terminal peptide; Epigenetic Research Peptide
EINECS	Contact for details

Quality Control

Every batch of Histone H3 (1-20) is manufactured under strict quality management systems. The product undergoes comprehensive analytical testing, including HPLC for purity verification and Mass Spectrometry (MS) for identity confirmation. We provide a detailed Certificate of Analysis (COA) with each shipment, ensuring traceability and compliance with research-grade specifications for consistent, reliable performance in sensitive applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. This peptide is hygroscopic (moisture-sensitive) and should be allowed to equilibrate to room temperature in a dry environment before opening to prevent condensation. Aliquotting is recommended to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC & MS)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Amino Acid Sequence	ARTKQTARKSTGGKAPRKQLA
Net Peptide Content	≥ 80%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.