Description

2,3-Bis(4-Methylphenyl)-5-(4-Cyanophenyl)Tetrazolium Chloride is a specialized tetrazolium salt derivative, a class of compounds renowned for their critical role as chromogenic and fluorogenic indicators in biochemical assays. This compound matters for its high sensitivity and reliability in detecting cellular metabolic activity, particularly in cell viability and cytotoxicity studies. It is primarily needed by research institutions, pharmaceutical R&D laboratories, and biotechnology companies engaged in high-throughput screening and drug discovery.

Application

• Cell Viability and Proliferation Assays: Used as a key substrate in MTT-like assays to quantify metabolically active cells.
• Cytotoxicity Screening: Essential for evaluating the toxic effects of novel drug candidates or chemical compounds on cultured cells.
• Microbiological Research: Applied in studies of microbial growth and metabolic activity.
• High-Throughput Screening (HTS): Serves as a robust endpoint reagent in automated drug discovery platforms.
• Apoptosis and Cell Death Studies: Helps in distinguishing between necrotic and apoptotic cell populations.
• Biomaterial and Implant Testing: Used to assess the biocompatibility of new materials by measuring cell health upon contact.

Basic Information

Product Name	2,3-Bis(4-Methylphenyl)-5-(4-Cyanophenyl)Tetrazolium Chloride
CAS No.	376591-03-6
Molecular Formula	C28H21ClN6
Molecular Weight	477.96 g/mol
Synonyms	5-(4-Cyanophenyl)-2,3-bis(4-methylphenyl)tetrazolium chloride; Tetrazolium Chloride, 2,3-bis(4-methylphenyl)-5-(4-cyanophenyl)-; Tetrazolium dye derivative; Cell proliferation assay reagent; MTT analog; Cytotoxicity assay reagent; Research biochemical 376591-03-6
EINECS	Contact for details

Quality Control

Our 2,3-Bis(4-Methylphenyl)-5-(4-Cyanophenyl)Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for sensitive research applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept sealed in a desiccated environment and handled under low-light conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.