Description

D-Ala-Lys-Amca CAS NO 375822-19-8 is a synthetic, fluorophore-labeled peptide derivative, specifically designed for advanced biochemical research and diagnostic applications. Its core value lies in its role as a highly sensitive and specific substrate for various proteolytic enzymes, including aminopeptidases and other serine proteases. This compound is essential for researchers and developers in the fields of pharmaceutical discovery, clinical diagnostics, and proteomics, where precise enzyme activity profiling and high-throughput screening are critical.

Application

• Enzyme Activity Assays: As a fluorogenic substrate for kinetic studies and inhibition profiling of aminopeptidases, dipeptidyl peptidases, and related enzymes.
• High-Throughput Screening (HTS): Used in drug discovery platforms to identify and characterize novel enzyme inhibitors for therapeutic targets.
• Protease Research: A critical tool for investigating protease specificity, function, and regulation in various biological pathways.
• Diagnostic Kit Development: Incorporated into in vitro diagnostic (IVD) reagents for the detection of specific enzyme activities associated with diseases.
• Biomarker Detection: Employed in assays designed to detect enzymatic biomarkers in research and clinical samples.
• Cell-Based Imaging: Potential use in live-cell imaging studies to localize and monitor protease activity in real-time.

Basic Information

Product Name	D-Ala-Lys-Amca
CAS No.	375822-19-8
Molecular Formula	C24H35N5O6
Molecular Weight	489.57 g/mol
Synonyms	D-Alanine-Lysine-AMC; H-D-Ala-Lys-AMC; D-Ala-Lys-7-Amino-4-methylcoumarin; Ala-Lys-AMC (D-isomer); L-Lysine, N2-[(7-amino-4-methyl-2-oxo-2H-1-benzopyran-3-yl)carbonyl]-N6-[(2R)-2-aminopropanoyl]-; AMCA-D-Ala-Lys; Fluorogenic substrate D-Ala-Lys-AMC; Aminopeptidase substrate D-Ala-Lys-AMC
EINECS	Contact for details

Quality Control

Our D-Ala-Lys-Amca is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, MS for identity confirmation, and NMR for structural verification, to ensure it meets the high standards required for sensitive research applications. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.