Description

n-Succinimidyl 6-(3-Maleimidopropionamido) Hexanoate is a heterobifunctional crosslinking reagent featuring an amine-reactive NHS ester and a thiol-reactive maleimide group. This compound matters for its ability to create stable, site-specific conjugates between proteins, peptides, antibodies, and other biomolecules, facilitating advanced research and therapeutic development. It is essential for scientists and manufacturers in bioconjugation, antibody-drug conjugate (ADC) development, and diagnostic assay production who require reliable and efficient crosslinking chemistry.

Application

• Antibody-Drug Conjugate (ADC) Linker: A critical component for covalently attaching cytotoxic payloads to monoclonal antibodies via lysine residues.
• Protein-Protein/Peptide Conjugation: Enables the creation of defined protein complexes, fusion proteins, and peptide-carrier protein conjugates for immunology and structural biology.
• Diagnostic Reagent Preparation: Used to label antibodies, enzymes (e.g., HRP), or other proteins with fluorescent dyes, haptens, or solid supports for ELISA and lateral flow assays.
• Surface Immobilization Chemistry: Functionalizes gold surfaces, chips, or nanoparticles with biomolecules for biosensor development and microarray technology.
• Polymer Modification: Introduces bioactive ligands or functional groups onto polymers and hydrogels for drug delivery systems and tissue engineering scaffolds.
• Stable Conjugate for In Vivo Studies: Produces bioconjugates with enhanced serum stability compared to cleavable linkers, suitable for pharmacokinetic and biodistribution studies.

Basic Information

Product Name	n-Succinimidyl 6-(3-Maleimidopropionamido) Hexanoate
CAS No.	367927-39-7
Molecular Formula	C17H23N3O7
Molecular Weight	381.38 g/mol
Synonyms	SMPH; Maleimide-PEG5-NHS ester; 6-((3-Maleimidopropanoyl)amino)hexanoic acid N-hydroxysuccinimide ester; N-Succinimidyl 6-((3-maleimidopropanoyl)amino)hexanoate; SM(PEG)5; NHS-PEG5-Maleimide; 6-Maleimidocaproyl-N-hydroxysuccinimide ester; N-(ε-Maleimidocaproyloxy)succinimide
EINECS	Contact for details

Quality Control

Our n-Succinimidyl 6-(3-Maleimidopropionamido) Hexanoate is manufactured under strict quality systems to ensure batch-to-batch consistency for sensitive bioconjugation applications. Each lot undergoes comprehensive analytical testing, including HPLC purity, NMR, and mass spectrometry for identity and impurity profile confirmation. Certificates of Analysis (COA) with detailed specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.