Description

H-Hyp(Tbu)-Otbu Hcl is a protected amino acid derivative, specifically a tert-butyloxycarbonyl (Boc) and tert-butyl (tBu) protected hydroxyproline hydrochloride salt. This compound is a critical building block in solid-phase peptide synthesis (SPPS), enabling the controlled assembly of complex peptides and proteins. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life sciences sectors who require high-purity intermediates for the development of therapeutic peptides, diagnostic agents, and biochemical tools.

Application

• Solid-Phase Peptide Synthesis (SPPS): As a key protected amino acid for the automated synthesis of peptide chains.
• Pharmaceutical R&D: Intermediate in the synthesis of peptide-based drug candidates and active pharmaceutical ingredients (APIs).
• Bioconjugation: Used to introduce hydroxyproline motifs into biomolecules for labeling or functionalization.
• Biochemical Research: A reagent for studying collagen structure, protein folding, and post-translational modifications.
• Combinatorial Chemistry: Building block for creating diverse peptide libraries for screening.
• Cosmeceutical Development: Potential intermediate in the synthesis of collagen-stimulating peptides for skincare applications.

Basic Information

Product Name	H-Hyp(Tbu)-Otbu Hcl
CAS No.	367453-05-2
Molecular Formula	C14H26N2O5 • HCl
Molecular Weight	338.83 g/mol (Free base: 302.37)
Synonyms	Fmoc-Hyp(tBu)-OH; (2S,4R)-1-(tert-Butoxycarbonyl)-4-(tert-butoxy)-L-proline hydrochloride; Boc-Hyp(OtBu)-OH • HCl; Boc-trans-4-Hydroxy-L-proline tert-butyl ester hydrochloride; trans-4-[(tert-Butoxycarbonyl)amino]-1-(tert-butoxycarbonyl)-L-proline hydrochloride; N-α-Boc-trans-4-hydroxy-L-proline tert-butyl ester hydrochloride; Protected Hydroxyproline HCl; 367453-05-2
EINECS	Contact for details

Quality Control

Our H-Hyp(Tbu)-Otbu Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis for enantiomeric excess, and NMR for structural confirmation, to ensure it meets the stringent requirements for peptide synthesis. Certificates of Analysis (COA) with detailed specifications are provided for every shipment, supporting compliance with cGMP and ICH guidelines for pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label or COA. This material is hygroscopic (moisture-sensitive) and should be handled under dry, inert conditions to maintain stability and purity. Keep container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0%
Chiral Purity (HPLC)	≥99.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.