Description

Ac-Nle-Pro-Nle-Asp-Amc CAS NO 355140-49-7 is a specialized fluorogenic peptide substrate designed for high-sensitivity enzymatic assays. This compound is critical for researchers and developers requiring precise measurement of protease activity, particularly in complex biological matrices. It is primarily utilized in pharmaceutical R&D, biochemical research, and diagnostic kit development for studying enzyme kinetics and inhibitor screening.

Application

• Protease Activity Assays: As a fluorogenic substrate for monitoring the activity of specific aspartyl proteases and related enzymes in real-time.
• Drug Discovery & High-Throughput Screening (HTS): Used in target-based screening platforms to identify and characterize novel protease inhibitors.
• Biochemical Research: For fundamental studies on enzyme mechanism, kinetics, and substrate specificity in purified systems.
• Diagnostic Development: A key component in the development of sensitive in-vitro diagnostic tests for disease biomarkers involving protease activity.
• Cell Biology Studies: Employed in cellular assays to detect and quantify intracellular protease activity under various physiological or pathological conditions.

Basic Information

Product Name	Ac-Nle-Pro-Nle-Asp-Amc
CAS No.	355140-49-7
Molecular Formula	C30H41N5O8
Molecular Weight	599.68 g/mol
Synonyms	Acetyl-Norleucyl-Prolyl-Norleucyl-Aspartyl-7-Amino-4-Methylcoumarin; Ac-Nle-Pro-Nle-Asp-AMC; N-Acetyl-Nle-Pro-Nle-Asp-AMC; Fluorogenic Peptide Substrate 355140-49-7; Aspartic Protease Substrate; Caspase Substrate Analog; AMC-labeled Peptide; (7-Amino-4-methylcoumarin)-conjugated Peptide
EINECS	Contact for details

Quality Control

Every batch of Ac-Nle-Pro-Nle-Asp-Amc is manufactured and tested under strict quality management protocols to ensure identity, purity, and performance consistency. Our products undergo rigorous quality testing, including advanced chromatographic and spectroscopic methods, to ensure compliance with research-grade standards. Certificates of Analysis (COA) detailing purity, HPLC profile, and other critical parameters are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.