Description

4-(Trifluoromethyl)Umbelliferyl Phosphate Disodium Salt is a high-purity, synthetic fluorogenic substrate essential for enzymatic detection and quantification. This compound matters for its role as a sensitive and specific reagent in biochemical assays, enabling precise measurement of phosphatase and other enzyme activities. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies engaged in drug discovery and molecular biology.

Application

• Fluorogenic Substrate for Enzymatic Assays: A key reagent for detecting and quantifying the activity of alkaline phosphatase (ALP), acid phosphatase, and other phosphomonoesterase enzymes.
• Diagnostic Kit Development: Used in the formulation of clinical diagnostic kits for measuring enzyme levels in biological samples, crucial for medical research and disease monitoring.
• High-Throughput Screening (HTS): Employed in pharmaceutical and biotechnology research for screening enzyme inhibitors or activators in drug discovery pipelines.
• Cell Biology & Histochemistry: Serves as a substrate in techniques for visualizing enzyme localization and activity within cells and tissue sections.
• Biomarker Research: Facilitates the study of enzyme-related biomarkers in various physiological and pathological processes.
• Quality Control in Biotechnology: Used to assay enzyme purity and activity during the production and purification of recombinant proteins.

Basic Information

Product Name	4-(Trifluoromethyl)Umbelliferyl Phosphate Disodium Salt
CAS No.	352525-17-8
Molecular Formula	C10H5F3Na2O7P
Molecular Weight	353.09 g/mol
Synonyms	4-Methylumbelliferyl-7-phosphate, Trifluoromethyl Derivative, Disodium Salt; 7-Hydroxy-4-(trifluoromethyl)-2H-1-benzopyran-2-one Dihydrogen Phosphate Disodium Salt; 4-Trifluoromethylumbelliferyl Phosphate, Disodium Salt; 4-TFMUP, Na2; 4-Trifluoromethyl-7-hydroxycoumarin Phosphate Disodium Salt; Fluorogenic Phosphatase Substrate; ALP Substrate, Trifluoromethyl Derivative
EINECS	Contact for details

Quality Control

Our 4-(Trifluoromethyl)Umbelliferyl Phosphate Disodium Salt is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR and IR for structural confirmation, and stringent controls for moisture and impurity content. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light may affect stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in water, DMSO
Fluorogenic Activity	Functional test passed

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.