Description

Enalaprilat-D5 (Phenyl-D5) is a deuterated internal standard of the active metabolite of the antihypertensive drug enalapril. This high-purity compound is essential for ensuring the accuracy and reliability of quantitative bioanalytical methods, particularly Liquid Chromatography-Mass Spectrometry (LC-MS/MS). Pharmaceutical research and development laboratories, clinical testing facilities, and contract research organizations (CROs) rely on this stable isotope-labeled standard for precise pharmacokinetic studies, drug metabolism research, and therapeutic drug monitoring.

Application

• Quantitative Bioanalysis: Serves as a critical internal standard for the accurate LC-MS/MS quantification of enalapril and enalaprilat in biological matrices (plasma, serum, urine).
• Pharmacokinetic/Pharmacodynamic (PK/PD) Studies: Enables precise measurement of drug concentration-time profiles to determine absorption, distribution, metabolism, and excretion (ADME) parameters.
• Clinical Research & Therapeutic Drug Monitoring (TDM): Used in assays to monitor patient drug levels, ensuring therapeutic efficacy and safety during hypertension or heart failure treatment.
• Drug Metabolism and Metabolite Identification: Aids in distinguishing drug-derived metabolites from endogenous compounds in mass spectrometry-based studies.
• Method Development and Validation: Provides a stable, non-interfering reference point for developing, optimizing, and validating robust analytical methods in compliance with GLP/GCP guidelines.
• Reference Standard for Impurity Testing: Can be utilized as a system suitability or identification standard in the quality control of active pharmaceutical ingredients (APIs).

Basic Information

Product Name	Enalaprilat-D5 (Phenyl-D5)
CAS No.	349554-00-3
Molecular Formula	C18H19D5N2O5
Molecular Weight	~ 353.42 g/mol
Synonyms	(2S)-1-[(2S)-2-[[(1S)-1-Carboxy-3-phenylpropyl]amino]propanoyl]pyrrolidine-2-carboxylic acid (phenyl-D5); Enalaprilat-d5; (S)-1-[N-[1-(Ethoxycarbonyl)-3-phenylpropyl]-L-alanyl]-L-proline (Phenyl-D5) diacid; Enalaprilat (phenyl-d5); Deuterated Enalaprilat; Enalaprilat Internal Standard; L-Proline, 1-[N-[1-(ethoxycarbonyl)-3-phenylpropyl-D5]-L-alanyl]-, (S)-; Enalapril metabolite-d5.
EINECS	Contact for details

Quality Control

Our deuterated standards are manufactured under strict quality control protocols to ensure the high chemical purity and isotopic enrichment required for sensitive analytical applications. Each batch is characterized by advanced spectroscopic and chromatographic techniques, including NMR, HPLC, and Mass Spectrometry. A comprehensive Certificate of Analysis (COA) is provided, detailing assay purity, isotopic purity (D-enrichment), and analytical results. Our quality system supports compliance with relevant pharmacopeial and ISO standards for reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) or as indicated on the label. This product is hygroscopic (moisture-sensitive). Keep the container tightly sealed in a dry environment to prevent degradation. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.